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Promoter sequences from the pathogenesis-related gene hin1 of Nicotiana tabacum and uses thereof

IP.com Disclosure Number: IPCOM000009443D
Publication Date: 2002-Aug-24
Document File: 2 page(s) / 217K

Publishing Venue

The IP.com Prior Art Database

Related People

Bob Newman: AUTHOR [+2]

Abstract

A plant defense response-inducible promoter was isolated from the tobacco hin1 gene. The hin1 promoter sequence was not available in public databases, though the cDNA was isolated previously (Gopalan S. et al., Plant J. 1996 Oct;10(4):591-600). The published coding sequence was used to isolate the hin1 promoter by 5’ RACE PCR. The hin1 promoter sequence is given below. The hin1 promoter may be useful for a number of approaches including, but not limited to plant pathogen resistance strategies, the construction of defense inducible reporter genes (e.g. defense inducible GFP), and plant based biological sensors. The utility of this promoter was shown in transgenic tobacco plants carrying the hin1 promoter fused to GFP.

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For publication on IP.com

Promoter sequences from the pathogenesis-related gene hin1 of Nicotiana tabacum and uses thereof

Bob Newman and Jim English

A plant defense response-inducible promoter was isolated from the tobacco hin1 gene. The hin1 promoter sequence was not available in public databases, though the cDNA was isolated previously (Gopalan S. et al., Plant J. 1996 Oct;10(4):591-600). The published coding sequence was used to isolate the hin1 promoter by 5’ RACE PCR. The hin1 promoter sequence is given below.

The hin1 promoter may be useful for a number of approaches including, but not limited to, the following examples:

(1)   Plant pathogen resistance strategies which depend on defense inducible promoters have been proposed. One such strategy would be to fuse the defense inducible promoter to a defense gene coding sequence. Upon pathogen attack, there would be strong localized induction of the defense gene which would prevent spread of the pathogen.

(2)   Another approach is to construct defense inducible reporter genes (e.g. defense inducible GFP). This may facilitate detecting induction of genes associated with pathogen defense and/or hypersensitive response. This would be useful for identification of plant disease resistance genes with altered recognition specificities.

(3)   Another potential use of defense-inducible reporter genes is plant based biological sensors. Upon exposure of the plant to a target biotic or abiotic agent, defense pathways would be turned on resulting in induction of the reporter gene.

The utility of thispromoter was shown in transgenic tobacco plants carrying the hin1 promoter fused to GFP. Tobacco line Xanthi, which carri...