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Biotransformation of Glycyrrhizin to Glycyrhizic acid monoglucuronide and Glycyrrhetic acid by Enzymes

IP.com Disclosure Number: IPCOM000011440D
Publication Date: 2003-Feb-20
Document File: 6 page(s) / 349K

Publishing Venue

The IP.com Prior Art Database

Abstract

Glycyrrhizin, which shows 170 times sweetness as sucrose, is the major natural sweet compound existing in licorice , and it has been used as the food-additive and medicine. Glycyrhizic acid monoglucurnide and glycyrrhizic acid are derivatives of Glycyrrhizin. The former one shows 1000 times sweetness as sucrose and the later one has better clinical effects, such as anti-inflammatory, analgesic and antiviral than the other two. VISCOZYME and GAMANASE, two commercial enzyme preparations from NOVOZYMES A/S, Krogshoejvej 36, DK-2880 Bagsvaerd, Denmark, can deglycosylate GL into GAMG and GA respectively at a high efficiency under gentle conditions. VISCOZYME is a multi-enzyme preparation obtained from Aspergillus aculeatus, it contains arabanase, cellulase, b-glucanase, hemicellulase, xylanase,pectinase and beta-glucuronidase activity. GAMANASE is a 1,4--D-Mannan mannanohydrolase obtained from Aspergillus niger. it also contained beta-glucuonidase activity.

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Biotransformation of Glycyrrhizin to Glycyrhizic acid monoglucuronide and Glycyrrhetic acid by Enzymes

Huang Hongzhi� � Xu Qing*� � Niels Elvig

Novozymes China

Abstract

Glycyrrhizin, which shows 170 times sweetness as sucrose, is the major natural sweet compound existing in licorice , and it has been used as the food-additive and medicine. Glycyrhizic acid monoglucurnide and glycyrrhizic acid are derivatives of Glycyrrhizin. The former one shows 1000 times sweetness as sucrose and the later one has better clinical effects, such as anti-inflammatory, analgesic and antiviral than the other two.� �

VISCOZYME and GAMANASE, two commercial enzyme preparations from NOVOZYMES A/S, Krogshoejvej 36, DK-2880 Bagsvaerd, Denmark, can deglycosylate GL into GAMG and GA respectively at a high efficiency under gentle conditions.

VISCOZYME is a multi-enzyme preparation obtained from Aspergillus aculeatus, it contains arabanase, cellulase, b-glucanase, hemicellulase, xylanase,pectinase and beta-glucuronidase activity.

� GAMANASE is a 1,4-b-D-Mannan mannanohydrolase obtained from Aspergillus niger. it also contained beta-glucuonidase activity.

Background

Glycyrrhizin (1, GL) is the major natural sweet compound existING in licorice, and it shows 170 times sweetness as sucrose and has been used as food-additive and medicine. Glycyrhizic acid monoglucurnide (3, GAMG) shows 1000 times sweetness as sucrose. Glycyrrhizic acid (2, GA) does not show sweetness, but its clinical effects, such as anti-inflammatory, analgesic and antiviral, are better than compounds 1 and 2.

To get compounds 2 and 3, the general method is hydrolyzing Glycyrrhizin because it is very difficult to synthesize them. Traditionally, Glycyrrhizin is hydrolyzed by HCl or H2SO4 , but the method has some disadvantages such as low yield, high danger for workers, and especially almost impossible to get compound 2 because there is no specificity for removing specific sugar by acid hydrolyzing. For these reasons, the GAMG market could not be developed well. Therefore, people have been searching new method to hydrolyze Glycyrrhizin. Recently, biotransformation methods have been developed. Some microorganisms can do the biotransformation have been found out. These results indicate that the biotransformation method is promising because of its high efficiency and specificity.

* Author to whom correspon...