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ANTIMICROBIAL POLYPEPTIDES

IP.com Disclosure Number: IPCOM000012281D
Publication Date: 2003-Apr-24

Publishing Venue

The IP.com Prior Art Database

Abstract

The present invention relates to polypeptides having antimicrobial activity and polynucleotides having a nucleotide sequence which encodes for the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid constructs as well as methods for producing and using the polypeptides.

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ANTIMICROBIAL POLYPEPTIDES

FIELD OF THE INVENTION

                    The present invention relates to polypeptides having antimicrobial activity and polynucleotides having a nucleotide sequence which encodes for the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid constructs as well as methods for producing and using the polypeptides.

BACKGROUND

                    It is an object of the present invention to provide polypeptides having antimicrobial activity and polynucleotides encoding the polypeptides.

SUMMARY

                    In a first aspect the present invention relates to a polypeptide having antimicrobial activity, selected from the group consisting of:

(a)             a polypeptide comprising an amino acid sequence which has at least 65% identity with amino acids 1 to 40 of SEQ ID NO:2;

(b)             a polypeptide comprising an amino acid sequence which has at least 65% identity with the polypeptide encoded by the antimicrobial polypeptide encoding part of the nucleotide sequence present in Pseudoplectania nigrella CBS 444.97;

(c)             a polypeptide which is encoded by a nucleotide sequence which hybridizes under low stringency conditions with a polynucleotide probe selected from the group consisting of:

(i)           the complementary strand of nucleotides 166 to 285 of SEQ ID NO:1,

(ii)           the complementary strand of nucleotides 70 to 285 of SEQ ID NO:1, and

(iii)          the complementary strand of nucleotides 1 to 285 of SEQ ID NO:1; and

(d)             a fragment of (a), (b) or (c) that has antimicrobial activity.

                    In a second aspect the present invention relates to polynucleotides having a nucleotide sequence which encodes for the polypeptide of the invention.

                    In a third aspect the present invention relates to a nucleic acid construct comprising the nucleotide sequence, which encodes for the polypeptide of the invention, operably linked to one or more control sequences that direct the production of the polypeptide in a suitable host.

                    In a fourth aspect the present invention relates to a recombinant expression vector comprising the nucleic acid construct of the invention.

                    In a fifth aspect the present invention relates to a recombinant host cell comprising the nucleic acid construct of the invention.

                    In a sixth aspect the present invention relates to a method for producing a polypeptide of the invention, the method comprising:

(a)                cultivating a strain, which in its wild-type form is capable of producing the polypeptide, to produce the polypeptide; and

(b)                recovering the polypeptide.

                    In a seventh aspect the present invention relates to a method for producing a polypeptide of the invention, the method comprising:

(a)                cultivating a recombinant host cell o...