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A Method for Normalization of Gene Expression Data and Detection of Expressed/Inhibited Genes

IP.com Disclosure Number: IPCOM000019913D
Original Publication Date: 2003-Oct-10
Included in the Prior Art Database: 2003-Oct-10
Document File: 2 page(s) / 63K

Publishing Venue

IBM

Abstract

Disclosed is a methodology for normalization of gene expression data and detection of expressed/inhibited genes, using an extended mode value of ratio of fluorescence densities of two microarrays. After each ratio value is converted to a distribution and all of the distributions are added, an extended mode value is obtained.

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  A Method for Normalization of Gene Expression Data and Detection of Expressed/Inhibited Genes

  In general, a microarray can measure gene expression levels as fluorescence densities with offset values.

Different microarrays have different offset values, normalization methodology is necessary (figure 1). Since expression levels of many genes could be constant, normalization is conducted based on the mode value of ratio of fluorescence densities of two microarrays (figure 2). After each ratio value is converted to a distribution and all of the distributions are added, the mode value for ratio values which are continuous quantity can be calculated.

Figure 1

Scatter graph of fluorescence densites

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Fluorescence densities of microarray II

Find an extended mode value of ratio of fluorescence densities

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Fluorescence densities of microarray I

Figure 2

Disclosed by International Business Machines Corporation

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