Publication Date: 2004-Apr-19
The IP.com Prior Art Database
A lipase variant as described in WO 00/60063 was fermented as known in the art. The fermentation broth was centrifuged, and ultra filtered.
0.1 % RODALON™ was added (as an 80% solution from stock source) to theultra filtered concentrate.
The conductivity was changed by adding a calcium solution (35%) from a stock source in Test B, but not in Test A (control). The samples were mixed for app. 30 minutes and sampled for TVC (total viable count) and activity (= yield).
Test Starting conductivity Conductivity after CaCl2 TVC Yield
A 2.5 mS/cm >250,000
B 2.25 mS/cm 8 mS/cm <10 100%
No additional precipitations were observed in Test B compared to Test A (Control).
It can be seen from the results above that TVC was reduced dramatically after the addition of calcium chloride.