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Enzymatic Production of Dihydroxyacetone

IP.com Disclosure Number: IPCOM000085738D
Original Publication Date: 1976-May-01
Included in the Prior Art Database: 2005-Mar-02
Document File: 1 page(s) / 12K

Publishing Venue

IBM

Related People

Studebaker, JF: AUTHOR

Abstract

Dihydroxyacetone may be synthesized enzymatically from glycerol, a starting material which is considerably cheaper than dihydroxyacetone itself. It is possible to accomplish the necessary recycling of the electron acceptor by the method discussed in an article by S.H. Slocum and J. F. Studebaker entitled "Protection of Enzymes During Recycling of Nicotinamide Adenine Dinucleotide (NAD/+/) With Phenazine Methosulfate (PMS) and O(2)", published in IBM Technical Disclosure Bulletin, Vol. 18, No. 12, May 1976, pages 4180 and 4181, or by other known recycling methods. Dihydroxyacetone (A) has a number of uses in the chemical industry: H O H (A) HO - C - C - C - OH H H H It can be produced by the oxidation of glycerol (B), a reaction:.

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Enzymatic Production of Dihydroxyacetone

Dihydroxyacetone may be synthesized enzymatically from glycerol, a starting material which is considerably cheaper than dihydroxyacetone itself. It is possible to accomplish the necessary recycling of the electron acceptor by the method discussed in an article by S.H. Slocum and J. F. Studebaker entitled "Protection of Enzymes During Recycling of Nicotinamide Adenine Dinucleotide (NAD/+/) With Phenazine Methosulfate (PMS) and O(2)", published in IBM Technical Disclosure Bulletin, Vol. 18, No. 12, May 1976, pages 4180 and 4181, or by other known recycling methods. Dihydroxyacetone (A) has a number of uses in the chemical industry: H O H (A) HO - C - C - C - OH H H H It can be produced by the oxidation of glycerol (B), a reaction:. H OH H (B) HOC - C - C - OH H H H which can be catalyzed by a bacterial enzyme requiring NAD/+/ as an electron acceptor: glycerol + NAD/+/ --> dihydroxyacetone + NADH + H/+/.

As is discussed in the above-identified article pages 4180 and 4181, it may be economically feasible to use NAD/+/ dependent enzymes in chemical reactions of this type in spite of the high cost of NAD/+/.

A recent catalog of Eastman Organic Chemicals/*/ indicates that dihydroxyacetone is considerably more expensive than glycerol. It follows that the cost of enzymatic production can be made competitive by using enzymatic catalysis with electron acceptor recycling to produce dihydroxyacetone from glycerol. * Supplement #1, date...