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Spectrophotometric Determination of Lactic Acid in Serum

IP.com Disclosure Number: IPCOM000086928D
Original Publication Date: 1976-Nov-01
Included in the Prior Art Database: 2005-Mar-03
Document File: 1 page(s) / 11K

Publishing Venue

IBM

Related People

Rich, DW: AUTHOR

Abstract

The most common method of analyzing for serum lactic acid is by ultraviolet spectrophotometry. This method requires not only a more expensive instrument, as opposed to visible spectrophotometry, but also more costly reagents.

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Spectrophotometric Determination of Lactic Acid in Serum

The most common method of analyzing for serum lactic acid is by ultraviolet spectrophotometry. This method requires not only a more expensive instrument, as opposed to visible spectrophotometry, but also more costly reagents.

A new visible method, for the determination of lactic acid, has been found. It essentially utilizes the ability of phosphotungstic acid to be reduced to tungsten blue. This latter product can be measured at 710 nanometers, and its color is proportional to the concentration of lactic acid in serum. The following reaction describes the analysis.

Lactic acid H(3)PW(12)O(40) ---> Pyruvic acid Tungsten blue.

Phosphotungstic acid oxidizes lactic acid to pyruvic acid and, in the process, it becomes quantitatively reduced to tungsten blue. Pyruvite interferes positively only if the ration of lactic to pyruvic acid is equal to or greater than 8.0. The percentage error is approximately 10% at such ratio.

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