Browse Prior Art Database

Viability Assay System

IP.com Disclosure Number: IPCOM000090866D
Original Publication Date: 1969-Aug-01
Included in the Prior Art Database: 2005-Mar-05
Document File: 2 page(s) / 28K

Publishing Venue

IBM

Related People

Kamentsky, LA: AUTHOR

Abstract

The System determines the viability ratio of live cells to dead cells using the standard trypan blue method of counting. The basic system employs only one photodetector for determining such viability ratio. Xenon lamp 2 passes light through an optical chamber comprising projection lens 4, aperture 6 and condenser lens 8 through area 10 of cell flow. The fluid under study passes through transparent tube T carrying the fluid and lying in the path of the light. Reflecting objective lens 12 is placed after area 10 and serves as a partial dark field lens. A 5900 angstroms interference filter 14 and condenser lens 16 image the focal plane of lens 12 onto photomultiplier tube 18.

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Viability Assay System

The System determines the viability ratio of live cells to dead cells using the standard trypan blue method of counting. The basic system employs only one photodetector for determining such viability ratio. Xenon lamp 2 passes light through an optical chamber comprising projection lens 4, aperture 6 and condenser lens 8 through area 10 of cell flow. The fluid under study passes through transparent tube T carrying the fluid and lying in the path of the light. Reflecting objective lens 12 is placed after area 10 and serves as a partial dark field lens. A 5900 angstroms interference filter 14 and condenser lens 16 image the focal plane of lens 12 onto photomultiplier tube 18. Aperture 20 of lens 16 is adjusted so that all but a small portion of the entrance cone of light entering the objective lens is obscured by the central mirror of lens 12. Threshold circuits 22 and 24 are coupled to counters 26 and 28. Operation of the system for determining the viability ratio is as follows. When a solution of trypan blue, ~
0.25% trypan blue in ~ 0.85% NaCl, is mixed with samples of blood containing live cells and dead cells, the dead cells become stained with the trypan blue and the live cells remain unstained. Trypan blue absorbs light having a frequency of 5900 angstroms. Consequently, when light enters the solution in the area of cell flow, the living cells scatter the incident light beam with insignificant absorption of light. The dead cells absorb...