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Leukocyte Assay

IP.com Disclosure Number: IPCOM000092061D
Original Publication Date: 1968-Aug-01
Included in the Prior Art Database: 2005-Mar-05
Document File: 1 page(s) / 11K

Publishing Venue

IBM

Related People

Kamentsky, LA: AUTHOR [+2]

Abstract

Lymphocytes and polymorphonucleated leukocytes PML in whole blood are rapidly assayed. Formalin fixed blood cells are doubly stained with the naphthal yellow s and feulgen reactions. The cell suspension is passed through a rapid cell analyzer fitted with a 25 micron channel illuminated with collimated light from a point source. Light is absorbed by the cells in the analyzer at wavelengths of 4300 angstroms and 5700 angstroms. These absorptions are estimates of total protein and desoxyribonucleic acid DNA per cell. Photographic displays produced at these wavelengths for normal blood show several distinct clusters of cell dots, two clusters of which represent the lymphocytes and PML in the blood sample. When blood from patients suffering with leukemia is assayed as above, one of the above cell clusters is not apparent.

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Leukocyte Assay

Lymphocytes and polymorphonucleated leukocytes PML in whole blood are rapidly assayed. Formalin fixed blood cells are doubly stained with the naphthal yellow s and feulgen reactions. The cell suspension is passed through a rapid cell analyzer fitted with a 25 micron channel illuminated with collimated light from a point source. Light is absorbed by the cells in the analyzer at wavelengths of 4300 angstroms and 5700 angstroms. These absorptions are estimates of total protein and desoxyribonucleic acid DNA per cell. Photographic displays produced at these wavelengths for normal blood show several distinct clusters of cell dots, two clusters of which represent the lymphocytes and PML in the blood sample. When blood from patients suffering with leukemia is assayed as above, one of the above cell clusters is not apparent. When cells from a culture of human epidermoid carcinogenic cells are added to the cell suspension, the epidermoid cells are displaced from the blood cells on the display by a factor of 2 to 3. The above stain combination is sensitive for detection of carcinogenic cells in blood.

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