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Browse Prior Art Database

Direct Analysis of Urea in Biological Fluids

IP.com Disclosure Number: IPCOM000092103D
Original Publication Date: 1968-Sep-01
Included in the Prior Art Database: 2005-Mar-05
Document File: 2 page(s) / 42K

Publishing Venue

IBM

Related People

Neff, GW: AUTHOR [+2]

Abstract

The system arrangement is for the direct analysis of urea in biological fluids. The system, which generates a continuous real-time indication of the urea parameter, has on-line capabilities with both a fluid source and a processing unit. These functional capabilities are achieved by providing an analysis system which acts nondestructively on small quantities of biological fluid without the addition of reagents or diluents to provide a direct measurement of urea concentration.

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Direct Analysis of Urea in Biological Fluids

The system arrangement is for the direct analysis of urea in biological fluids. The system, which generates a continuous real-time indication of the urea parameter, has on-line capabilities with both a fluid source and a processing unit. These functional capabilities are achieved by providing an analysis system which acts nondestructively on small quantities of biological fluid without the addition of reagents or diluents to provide a direct measurement of urea concentration.

A biological fluid enters and leaves sample cavity 3 by way of tubes 1 and 2 respectively. Semipermeable membrane 4 separates cavity 3 from reaction chamber 5. The latter contains the enzyme urease to react with urea selectively separated from fluid by membrane 4. The reaction produces carbon dioxide and ammonia in amounts proportional to the urea concentration.

Membrane 7 allows passage of ammonia gas molecules to electrolytic detection apparatus 8 but prevents passage of ions. Hence, there is no exchange of ions between the liquid enzyme layer and the electrolyte inside detection device 8. Washer 6 separates membranes 4 and 7 and, further, confines the enzyme reaction region to its central opening.

Detection device 8 includes a platinum anode and cathode 9 and 10, respectively, and a common reference electrode 11. Narrow channel 12, containing a buffered electrolytic bromide solution 13, communicates anode 9 with cathode 10. The bromide solution...