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Blood Cell Washing

IP.com Disclosure Number: IPCOM000092382D
Original Publication Date: 1967-Dec-01
Included in the Prior Art Database: 2005-Mar-05
Document File: 2 page(s) / 70K

Publishing Venue

IBM

Related People

Jones, AL: AUTHOR

Abstract

The centrifuge arrangement is especially suited for washing blood cells. Centrifuge bowl 1 is provided with cover 3, solid core 5, and flexible membrane 7. The space between core 5 and membrane 7 can be filled with a suitable liquid such as water indicated at 9. Such filling is effected by a passage which extends through core 5, through drive mechanism 11 of the centrifuge housing and through permanent seal 13 to pump 15 and water reservoir 17. Pump 15 is reversible. The volume of water contained within the membrane 7 can be altered by pumping water in or out of the space by pump 15. Thus the effective volume of the centrifuge can be controllably varied. Also disposed within the centrifuge chamber is blood bag 19 of suitable flexible material, containing the blood to be washed.

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Blood Cell Washing

The centrifuge arrangement is especially suited for washing blood cells. Centrifuge bowl 1 is provided with cover 3, solid core 5, and flexible membrane 7. The space between core 5 and membrane 7 can be filled with a suitable liquid such as water indicated at 9. Such filling is effected by a passage which extends through core 5, through drive mechanism 11 of the centrifuge housing and through permanent seal 13 to pump 15 and water reservoir 17. Pump 15 is reversible. The volume of water contained within the membrane 7 can be altered by pumping water in or out of the space by pump 15. Thus the effective volume of the centrifuge can be controllably varied. Also disposed within the centrifuge chamber is blood bag 19 of suitable flexible material, containing the blood to be washed.

Blood cells BC are forced to the wall of bag 19 and the centrifuge wall by the centrifugal force occurring during rotation of bowl 1. Supernatant SN is nearer the center of bag 19. Communication with bag 19 is effected by a temporary seal 21. The latter enables a connection to be made via valves V1 and V2 to a source of blood to be washed or inlet wash solutions via V1 or via V2 to waste receiver 23. The drive mechanism connected to pulley 11 is arranged to either spin the centrifuge head at a constant speed or to oscillate the head in an agitation cycle.

To wash cells BC, bag 19 is placed in the centrifuge, either already filled with blood or filled via V1. Then the cen...