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Automatic Dilution System for Cell Analyzer

IP.com Disclosure Number: IPCOM000093294D
Original Publication Date: 1967-Aug-01
Included in the Prior Art Database: 2005-Mar-06
Document File: 2 page(s) / 33K

Publishing Venue

IBM

Related People

Brown, RD: AUTHOR

Abstract

The analyzer measures and displays multiple spectrophotometric properties of biological cells in liquid suspension at high rates. The cells to be analyzed are suspended in a dense solution in container 14 from which they are fed to the analyzer. In order to insure that both the cell rate and volume flow rate through the analyzer are within the proper ranges, a servo control system is used. A dense cell suspension is fed from container 14 through pump 16 and mixed with a buffer. The latter is pumped by pump 18 from container 19. The operation of these two pumps is controlled by servo motor 20 to maintain the proper ratio of buffer to cells in the solution fed to analyzer 10 and, at the same time, maintain the proper volume flow rate. The cells are detected in analyzer 10. The average rate, i.e.

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Automatic Dilution System for Cell Analyzer

The analyzer measures and displays multiple spectrophotometric properties of biological cells in liquid suspension at high rates. The cells to be analyzed are suspended in a dense solution in container 14 from which they are fed to the analyzer. In order to insure that both the cell rate and volume flow rate through the analyzer are within the proper ranges, a servo control system is used. A dense cell suspension is fed from container 14 through pump 16 and mixed with a buffer. The latter is pumped by pump 18 from container 19. The operation of these two pumps is controlled by servo motor 20 to maintain the proper ratio of buffer to cells in the solution fed to analyzer 10 and, at the same time, maintain the proper volume flow rate. The cells are detected in analyzer 10. The average rate, i.e., cells/sec, is determined by cell rate meter 26. The output of meter 26 is applied to null detector and servo amplifier circuit 30. The other input to circuit 30 is derived from cell rate reference 32. Circuit 30 provides an output which depends upon the relationship between the actual cell rate and the reference cell rate. This output is applied to servo motor 20 which controls pumps 16 and 18 to achieve the desired cell and volume flow through analyzer 10. Provision can also be made for inhibiting operation of analyzer 10 when the cell rate is not within the proper range.

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