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Browse Prior Art Database

Fluorescence Attachment for Spectrophotometers

IP.com Disclosure Number: IPCOM000095622D
Original Publication Date: 1964-Mar-01
Included in the Prior Art Database: 2005-Mar-07
Document File: 2 page(s) / 31K

Publishing Venue

IBM

Related People

Hardy, WA: AUTHOR

Abstract

A commercially available double beam spectrometer is modified to increase its application to fluorescent analysis. Most double beam spectrophotometers that are primarily intended for absorption analysis employ a source of light, visible or infrared. The latter is divided into two beams by mirrors M(1) and M(2), each beam being chopped or modulated by rotating plates C, ultimately passing by means of a series of mirrors and lenses as two beams through a compartment D. Compartment D contains a standard absorbing medium and a sample holder for supporting a sample absorbing medium under tests. The two beams exit from the sample holder through openings O and O', such beams going to suitable analyzing equipment.

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Fluorescence Attachment for Spectrophotometers

A commercially available double beam spectrometer is modified to increase its application to fluorescent analysis. Most double beam spectrophotometers that are primarily intended for absorption analysis employ a source of light, visible or infrared. The latter is divided into two beams by mirrors M(1) and M(2), each beam being chopped or modulated by rotating plates C, ultimately passing by means of a series of mirrors and lenses as two beams through a compartment
D. Compartment D contains a standard absorbing medium and a sample holder for supporting a sample absorbing medium under tests. The two beams exit from the sample holder through openings O and O', such beams going to suitable analyzing equipment.

It is convenient to use this type of instrument as a monochromator, using the electronics, light gathering systems, mirrors, etc., of the conventional double beam spectrophotometer. The use of the device as a monochromator for fluorescent analysis without the addition of auxiliary equipment is accomplished in the following manner. The source is replaced by mirror M and the actual source of light to be used for fluorescent analysis, which is a source of ultraviolet rays, is placed within the sample compartment at location B. The sample being analyzed is placed within the sample compartment D somewhere within the path of light L. Mirror M is placed in the path of the ultraviolet light source B so that a chopped beam...