Technology for the Rapid Isolation of Circular Nucleic Acid Using a Thin Layers and Applied Electric Fields.
Original Publication Date: 2001-May-10
Included in the Prior Art Database: 2005-Jun-09
National Institute of Standards and Technology
This invention describes a technology that permits the rapid and specific isolation of circular nucleic acids. An electric field is used to transport the nucleic acids into a thin layer (also referred to as a membrane), that selectively retains the circular nucleic acids. Linear nucleic acids and other contaminants are not retained and pass through the thin layer. Very selective separations can be accomplished by controlling the physical properties and chemical composition of the thin layer and by varying the applied electric field. The separations are very rapid because the migration distances are small. The use of a thin layer allows the electric field to be used efficiently and allows rapid heat removal. The technology, described in this invention, can be scaled to process many samples or the surface area of the thin layer increased to process large volumes of a single sample.