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Postnatal development of brainstem cholinergic inputs to the dorsal lateral geniculate nucleus of the domesticated ferret, Mustela putorius furo

IP.com Disclosure Number: IPCOM000128053D
Original Publication Date: 1998-Dec-31
Included in the Prior Art Database: 2005-Sep-14
Document File: 4 page(s) / 19K

Publishing Venue

Software Patent Institute

Related People

Hitz, Sherri Lynn: AUTHOR [+3]

Related Documents

http://theses.mit.edu:80/Dienst/UI/2.0/Describe/0018.mit.theses/1998-205: URL

Abstract

The ferret dorsal lateral geniculate nucleus (cILGN) undergoes two periods of retinal afferent segregation during postnatal development. The first establishes the eye-specific laminae, A and Al, and the second establishes ON/OFF sublaminae within laminae A and Al. In contrast to eyespecific segregation, which seems to rely only on presynaptic activity, ON/OFF sublanunation requires both pre- and postsynaptic activity. Because of its dependence on postsynaptic (relay cell) activity, sublarnination may be influenced by extraretinal inputs which alter relay cell excitability. We have examined the postnatal development of cholinergic bramstem inputs to the dLGN to determine whether these inputs arrive in time to influence sublamination and whether the cholinergic innervation is present in laminar zones (i.e., whether it might target relay cells). Choline acetyltransferase (ChAT) immunoreactivity is not detected in the dLGN until a few days after the second postnatal week (just after sublamination begins), at which time it can be seen in both A and C laminae. ChAT labeling increases in intensity until two days before the end of the fourth postnatal week (when ON /OFF sublamination is cornp!cte), when it drops dramatically throughout the dLGN . CELAT labeling returns a few days later, but appears in the interlaminar and intersublaminar zones instead of within the A and C laminae. However, the pattern of ChAT labeling reverses once more, so that in the adult, ChAT labeling appears in the A and C laminae and is relatively absent from interlaminar zones. Acetylcholinesterase (ACNE) labeling in the dLGN shows a similar ontogenetic pattern and time course. Rettograde labeling of brainstem cholinergic nuclei demonstrates that these inputs are in place in the dLGN after the second postnatal week. Thus, cholinergic inputs to A and A1 laminae of the ferret dLGN do arrive in time to influence ON /OFF sublamination. Thesis Supervisor: Mriganka Sur Title: Fairchild Professor of Neuroscience 3 4

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Postnatal Development of Brainstem Cholinergic Inputs to the Dorsal Lateral Geniculate Nucleus of the Domesticated Ferret, Mustela putorius furo

by

Sherri Lynn Hit?

B.S., Biology B.A., French Cleveland State University 1993 Submitted in partial fulfillment of the requirements for the degree of MASTER OF SCIENCE in BRAIN AND COGNITIVE SCIENCES

at the Massachusetts Institute of Technology

SEPTEMBER 1998
SIGNATURE OF author: [[signature omitted]]

Department of Brain and Cognitive Sciences

August 8, 1998

CERTIFIED BY: [[SIGNATURE OMITTED]]

Mriganka Sur Fairchild Professor of Neuroscience Thesis Supervisor ACCEPTED BY: [[SIGNATURE OMITTED]]

Gerald Schneider Professor of Neuroscience Chairman, Department Graduate Committee ARCHIVES MASSACHUSETTS INSTITUTE OF TECHNOLOGY LIBRARIES SEP 21 1998

Massachusetts Institute of Technology Page 1 Dec 31, 1998

Page 2 of 4

Postnatal development of brainstem cholinergic inputs to the dorsal lateral geniculate nucleus of the domesticated ferret, Mustela putorius furo

Postnatal Development of Brainstem Cholinergic Inputs to the Dorsal Lateral Geniculate Nucleus of the Domesticated Ferret, Mustela putofius furo

by

Sherri Lynn Hitz

Submitted to the Department of Brain and Cognitive Sciences on August 8, 1998 in Partial Fulfillment of the Requirements for the Degree of Master of Science in Brain and Cognitive Sciences

ABSTRACT

The ferret dorsal lateral geniculate nucleus (cILGN) undergoes two periods of retinal afferent segregation during postnatal development. The first establishes the eye-specific laminae, A and Al, and the second establishes ON/OFF sublaminae within laminae A and Al. In contrast to eyespecific segregation, which seems to rely only on presynaptic activity, ON/OFF sublanunation requires both pre- and postsynaptic activity. Because of its dependence on postsynaptic (relay cell) activity, sublarnination may be influenced by extraretinal inputs which alter relay cell excitability. We have examined the postnatal development of cholinergic bramstem inputs to the dLGN to determine whether these inputs arrive in time to influence sublamination and whether the cholinergic innervation is present in laminar zones (i.e., whether it might target relay cells). Choline acetyltransferase (ChAT) immunoreactivity is not detected in the dLGN until a few days after the second postnatal week (just after sublamination begins), at which time it can be seen in both A and C laminae. ChAT labeling increases in intensity until two days before the end of the fourth postnatal week (when ON /OFF sublamination is cornp!cte), when it drops dramatically throughout the dLGN . CELAT labeling returns a few days later, but appears in the interlaminar and intersublamin...