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METHOD FOR PACKING CHROMATOGRAPHY COLUMNS

IP.com Disclosure Number: IPCOM000198702D
Publication Date: 2010-Aug-12
Document File: 2 page(s) / 31K

Publishing Venue

The IP.com Prior Art Database

Abstract

In the inventive cyclic axial packing method the bed is mechanically compressed in steps with a flow conditioning in between. The standard direct packing is performed to a lower packing factor (compression) than the target packing factor. Then the flow conditioning is performed. The bed is further compressed and then flow conditioned again. This is repeated until the target packing factor (compression) is reached. In the inventive flow packing method the media is first consolidated at a predefined liquid velocity by lowering the adaptor and keeping the column bottom mobile phase open. The pre defined liquid velocity giving the optimal compression is depending on for example media, bed height and diameter of the column. The adapter movement is stopped at the consolidated bed height or just before the consolidated bed height.

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METHOD FOR PACKING CHROMATOGRAPHY COLUMNS

The present invention relates to packing of chromatography columns with a cyclic procedure, including mechanical compression in steps with flow conditioning in between. This new packing method will remove test peak shoulder for certain chromatography media.

In a standard axial packing of chromatography columns, the chromatography medium is compressed by the adaptor in one step, from the consolidated bed height directly to the packed bed height. Cyclic packing has previously been discussed in flow or pressure packing.

This method does not seem to work for all medium. One example where direct packing may not work is for certain types of polishing media. When packing with this method to the desired packing factor a shoulder is seen in the efficiency test, see fig 1. One explanation for the shoulder is that the bed cracks and small channels are present in the bed. This shoulder is not seen when packing to a lower packing factor but in these cases the possible operational velocity is too low. Sometimes shoulders like this can be removed by performing a flow conditioning after packing the bed directly to the target compression. However this seems not possible for some media.

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u2356014test001:HETPUpFlow_UV1:131_280nm@01,BASEM

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Fig 1. Example polishing media packed to 19.7 cm bed height with a standard direct packing method to packing factor 1.15.

Using a cyclic axial packing method solve this problem, the desired packing factor can be received to get optimal pressure/flow and the efficiency test gives a nice peak.

In the cyclic axial packing method the bed is mechanically compressed in steps with a flow conditioning in between. The standard direct packing is performed to a lower packing factor (compression) than the target packing factor. Then the flow conditioning is performed. The bed is further compressed and then flow conditioned again. This is repeated until the target packing factor (compression) is reached.

In the tested method for the example polishing media the flow conditioning was run downflow through the bed for about 30 minutes. The flow was increased in steps like when running a traditional Pressure/flow curve. However, one can assume that the highest flow velocity can be run directly to achieve the same effect of flow conditioning. The compression/flow conditioning was repeated 1-2 times to achieve the target bed height. No shoulder was seen, see fig 2.

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