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Sterile Liners for Stainless Steel Stirred Tank Bioreactors

IP.com Disclosure Number: IPCOM000214914D
Publication Date: 2012-Feb-13
Document File: 3 page(s) / 54K

Publishing Venue

The IP.com Prior Art Database

Abstract

A sterile and disposable liner with magnetic coupled impellers as well as connectors for easy addition/harvest/sampling will be useful for facilities that have conventional stainless steel (SS) bioreactors and want to achieve the advantages of single-use technology without changing to an entirely new disposable bioreactor facility.

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Sterile Liners for Stainless Steel Stirred Tank Bioreactors

A sterile and disposable liner with magnetic coupled impellers as well as connectors for easy addition/harvest/sampling will be useful for facilities that have conventional stainless steel (SS) bioreactors and want to achieve the advantages of single-use technology without changing to an entirely new disposable bioreactor facility.

With this liner the user can easily install a disposable liner into the SS bioreactor and get all the benefits that come with the single-use bioreactor and still be able to use the same control system and media supply that are connected to the SS bioreactor. The liner concept can be used with bioreactors of e.g. 10 - 2000 L volume.

The liner can e.g. be constructed as illustrated below. It is similar to a normal single-use stirred tank bioreactor bag but instead of having it installed in a specific container it is inserted into a SS bioreactor. First the impellers are removed from the agitator shaft on the SS bioreactor. Then magnets are attached to the shaft and tightened using e.g. an Allen key. The liner is then placed inside the SS bioreactor and the agitator shaft with the magnets on it is aligned inside the bag's sleeve. The magnets on the shaft then control the stirrer speed of the impellers on the inside of the bag like it is usually done in typically shaft driven single-use bioreactor. Addition lines and connectors may be aligned with the ports on the SS bioreactor but since there is no industrial standard for the positioning of the ports it might be difficult to standardize these openings on the bag. An alternative is thus that sampling and addition/harvest is done from a...