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LIQUID CHROMATOGRAPHY WITH ULTRASONICALLY ENHANCED PERFORMANCE

IP.com Disclosure Number: IPCOM000225000D
Publication Date: 2013-Jan-18
Document File: 4 page(s) / 148K

Publishing Venue

The IP.com Prior Art Database

Abstract

Ultrasound is applied over a separation column during the chromatographic separation to accelerate the mass transport of sample molecules. Ultrasound can also be utilized for desorption and elution or cleaning-in-place (CIP) with ultrasound.

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Page 01 of 4

LIQUID CHROMATOGRAPHY WITH ULTRASONICALLY ENHANCED PERFORMANCE

Abstract

Ultrasound is applied over a separation column during the chromatographic separation to accelerate the mass transport of sample molecules. Ultrasound can also be utilized for desorption and elution or cleaning-in-place (CIP) with ultrasound.

Background and problem

The high molecular mass of proteins results in them having much lower diffusion coefficients than small molecules. A solution to overcome pore-diffusion effects of large molecules would have a tremendous impact on the performance of bioseparation and would result in a quantum leap in life science separation.

Technical concept and solution

It is suggested to test an external field of ultrasound applied over the separation column during the chromatographic separation to accelerate the mass transport of sample molecules. Ultrasound can also be utilized for desorption and elution or cleaning-in-place (CIP) with ultrasound may also be possible.

Ultrasound has already found numerous applications in the field of sonochemistry. In chromatography it has been shown that ultrasound can accelerate mass transport, enhance desorption and increase the adsorption capacity. The frequencies and intensities have to be optimized for different physicochemical effects.

Reference is made to Brandon S. Schueller and Ralph T. Yang. Ind. Eng. Chem. Res, 40 (2001), 4912-4918 where the effects of ultrasound on the adsorption and desorption of phenol using activated carbon and polymeric resin are investigated. For adsorption in a batch adsorber, ultrasound was found to act like a mixer, improving the mass-transfer coefficient through cavitation and acoustic streaming. For desorption ultrasound was found to enhance the surface diffusivity.


Page 02 of 4

The growth and implosion of bubbles at ultrasound treatment, where the implosion of bubbles results in a liquid jet that can move up to speeds of 400 km/h, is illustrated below.

It is suggested to test the above concept by applying an external field of ultrasound (with different frequencies and intensities) in the direction perpendicular to the longitudinal axis of the column as shown in the schematic views of ultrasound enhanced chromatography schematically illustrated below. As also il...