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POLYPEPTIDES HAVING ALPHA AMYLASE ACTIVITY

IP.com Disclosure Number: IPCOM000236237D
Publication Date: 2014-Apr-14
Document File: 197 page(s) / 1M

Publishing Venue

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POLYPEPTIDES HAVING ALPHA AMYLASE ACTIVITY

REFERENCE TO A SEQUENCE LISTING

        This application contains a Sequence Listing in computer readable form, which is 5 incorporated herein by reference.

BACKGROUND OF THE INVENTION Field of the Invention

         The present invention relates to alpha-amylases (polypeptides having alpha-amylase 10 activity), nucleic acids encoding the alpha-amylases, methods of producing the alpha-amylases, compostitions comprising the alpha-amylases and methods of using the alpha-amylases.

Description of the Related Art

         Alpha-amylases (alpha-1,4-glucan-4-glucanohydrolases, E.C. 3.2.1.1) constitute a group 15 of enzymes, which catalyses hydrolysis of starch and other linear and branched 1,4-gluosidic oligo- and polysaccharides.

         There is a long history of industrial use of alpha-amylases in several known applications such as detergent, baking, brewing, starch liquefaction and saccharification e.g. in preparation of high fructose syrups or as part of ethanol production from starch. These and other 20 applications of alpha-amylases are known and utilize alpha-amylases derived from microorganisms, in particular bacterial alpha-amylases.

     Among the first bacterial alpha-amylases to be used were alpha-amylases from B. amylolichefaciens also known as BAN® and from B.licheniformis, also known as Termamyl®.

    Termamyl has been extensively characterized and the crystal structure has been determined for 25 this enzyme. Bacillus amylases, such as BAN, Termamyl, AA560 (WO 2000/060060) and

     SP707 (described by Tsukamoto et al., 1988, Biochem. Biophys. Res. Comm. 151: 25-31) form a particular group of alpha-amylases that have found use in detergents. These amylases have been modified to improve the stability in detergents. WO 96/23873 e.g. disclose to delete the amino acids 181+182 or the amino acids 183+184 of SP707 (SEQ ID NO: 7 of WO 96/23873) to 30 improve the stability of this amylase. WO 96/23873 further disclose to modify the SP707 amylase by substituting M202 with e.g. a leucine to stabilize the molecule towards oxidation. Thus, it is known to modify amylases to improve certain properties. Most recently, for environmental reasons, it has become increasingly important to lower the temperature in washing, dishwashing and/or other cleaning processes. Despite the efficiency of current 35 detergent enzyme compositions, there are many stains that are difficult to completely remove not least due to the increased use of low (e.g., cold water) wash temperatures and shorter washing cycles. Thus, there is a need for amylolytic enzymes that can function under low temperature and at the same time preserve or increase desirable properties of the alpha-

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amylase, such as specific activity (amylolytic activity), stability, stain removal effect and/or wash performance.

         Thus, it is an object of the present invention to provide polypeptides having alpha- amylase activity (alpha-amylases) which have high performance, in particu...