METHOD FOR SOLID-PHASE EXTRACTION AND RELATED SYNTHETIC METHODOLOGIES
Publication Date: 2014-Jun-10
The IP.com Prior Art Database
Solid-phase extraction (SPE) is a separation or purification process by which compounds that are dissolved or suspended in a liquid mixture are separated from other compounds in the mixture according to their physical and chemical properties. SPE generally involves trapping a mixture on a sorbent and then eluting the desired component. This can be labor intensive, solvent intensive and not easily adapted to automate.
The method disclosed herein simplifies the SPE process by removing the need to filter, magnetically stir or use other methods of isolation of analyte or reaction material, thereby simplifying the SPE process for the user. This method can be adapted to accommodate existing equipment and instruments, ensuring broad applicability.
The disclosed method improves on certain aspects of existing technology as applied to particle formation processes for use as discrete SPE entities that can be utilized in a simplified format or as solid phase synthetic particles (for example by the method of WO 2005/056169A1 by Melin, et al.).
In essence, the beads so formed are too large (ca. 2 mm, but variable if required) to enter into a pipette / syringe and can be provided, for example, in vials into which an analyte solution is passed. The suspension can then be either agitated or not as defined by testing. Agitation can be as simple as repeat in/out pipetting of sample onto separation media or by traditional or automated means or not at all, as needed. The sample analyte can then be drawn into a syringe while the solation medium and extracted fraction are left in the vial. In another embodiment, the analyte can be trapped on the bead and released by elution with a different solvent. Examples of bead technology can be based on SPE process silica or by polystyrene based or other technologies.
Exemplary applications of the disclosed method include use in connection with organic synthesis in eliminating filtration steps. This technique can also have utility in Molecularly Imprinted Polymers (MIPs), Ion Exchange Chromatography (IEX) or immunosorbent purification.
Non-shedding particles are a prerequisite of this technique. Removal of eluent / reaction solution from the bead(s) container can be optimized. This can be, for example, drawing liqu...