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POLYPEPTIDES HAVING XYLANASE ACTIVITY AND POLYNUCLEOTIDES ENCODING THE SAME

IP.com Disclosure Number: IPCOM000239824D
Publication Date: 2014-Dec-03

Publishing Venue

The IP.com Prior Art Database

Abstract

The present invention relates to isolated polypeptides having xylanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

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POLYPEPTIDES HAVING XYLANASE ACTIVITY AND POLYNUCLEOTIDES ENCODING THE SAME           

BACKGROUND OF THE INVENTION

1. Field of the Invention

[001]       The present invention relates to isolated polypeptides having xylanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

2. Description of the Related Art

[001]       Plant cell wall polysaccharides constitute approximately 90% of the plant cell wall and can be divided into three groups: cellulose, hemicellulose, and pectin. Cellulose represents the major constituent of call wall polysaccharides. Hemicelluloses are the second most abundant constituent of plant cell walls. The major hemicellulose polymer is xylan. The structure of xylans found in cell walls of plants can differ significantly depending on their origin, but they always contain a beta-1,4-linked D-xylose backbone. The beta-1,4-linked D-xylose backbone can be substituted by various side groups, such as L-aribinose, D-galactose, acetyl, feruloyl, p-coumaroyl, and glucuronic acid residues.

[002]       The biodegradation of the xylan backbone depends on two classes of enzymes: endoxylanases and beta-xylosidases. Endoxylanases (EC 3.2.1.8) cleave the xylan backbone into smaller oligosaccharides, which can be further degraded to xylose by beta-xylosidases (EC 3.2.1.37).  Other enzymes involved in the degradation of xylan include, for example, acetylxylan esterase, arabinase, alpha-glucuronidase, ferulic acid esterase, and p-coumaric acid esterase.

[003]       Merchant et al., 1988, Biotechnology Letters 10: 513-516, describe the production of xylanase by Thielavia terrestris. Gilbert et al., 1993, Applied Microbiology and Biotechnology 40: 508-514, disclose a comparison of two xylanases from Thielavia terrestris and Thermoascus crustaceus. WO 1997/027293 discloses an enzyme from Thielavia terrestris exhibiting xylanase activity.

[004]       The present invention relates to polypeptides having xylanase activity and polynucleotides encoding the polypeptides.

SUMMARY OF THE INVENTION

[005]       The present disclosure relates to isolated polypeptides having xylanase activity and isolated polynucleotides encoding the polypeptides. The disclosure also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. The disclosure also pertains to a composition comprising such polypeptides and the method of using the composition.

[006]       In one aspect, the present disclosure provides an isolated, synthetic, or recombinant polypeptide having xylanase activity, selected from the group consisting of: a) a polypeptide comprising an amino acid sequence having at least 60% identity to the mature polypeptide of SEQ ID NO: 4, 8, 12, 16, 20, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 9...