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Tool for Controlling Fluid Flow in Multi-Well Chromatography Apparatus and Use Thereof

IP.com Disclosure Number: IPCOM000240312D
Publication Date: 2015-Jan-21
Document File: 6 page(s) / 311K

Publishing Venue

The IP.com Prior Art Database

Abstract

Improvements have been made to multi-well chromatography apparatus systems for gravitational and pressurized flow and collection of fluids through a porous media. The equipment described successfully abates sealing and leak issues involved with conventional methods of sealing multi-well chromatography or filter-style plates, such as sealing mats, and provides a method for control of flow and flow rates for the plate without increasing backpressure from the outlet of the columns. This customizable, valved manifold design is useful in a variety of liquid or gas chromatography applications in areas such as oil and gas chemistry, chemical engineering, biotechnology, molecular biology, microbiology, pharmaceutical development and academic research.

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Page 01 of 6

Tool for Controlling Fluid Flow in Multi-Well Chromatography Apparatus and Use Thereof

Abstract

Improvements have been made to multi-well chromatography apparatus systems for gravitational and pressurized flow and collection of fluids through a porous media. The equipment described successfully abates sealing and leak issues involved with conventional methods of sealing multi-well chromatography or filter-style plates, such as sealing mats, and provides a method for control of flow and flow rates for the plate without increasing backpressure from the outlet of the columns. This customizable, valved manifold design is useful in a variety of liquid or gas chromatography applications in areas such as oil and gas chemistry, chemical engineering, biotechnology, molecular biology, microbiology, pharmaceutical development and academic research.

Introduction

Currently, the flows of fluids in individual columns of high throughput column chromatography assays are controlled through the use of silicone-PTFE sealing mats, or are expedited through the use of vacuum or centrifugation. Fluid in this paper is described as any substance that obeys fluid mechanic or dynamic principles, or continually deforms or flows under an applied shear stress. The sealing is necessary for two purposes in some assays: 1) to prohibit the flow of fluids during column loading or incubation steps and 2) to temporarily (and reversibly) stop the flow of fluids in order to take displacement measurements or fraction collections. Unfortunately, the current method of controlling flow allows leaks (resulting in errors, lost time and waste of materials) and requires pulling or pressing actions which cause wear and tear on the sealing mats. The purpose of the described invention is to facilitate the control (prohibiting/allowing) of fluid flow of multiple wells of our multi-well chromatography/filter plates at the same time without leaks in order to decrease error and increase reproducibility of high-throughput systems while allowing for a user-friendly, reliable and efficient design.

Description

Application Background. High throughput multi-well chromatography assays may require fraction collection at various time points of sample liquids which flow out of the column or filter plate with time to measure fluid displacement rates to collect samples for analysis. Traditionally, we have stopped/started the flow of fluids in these columns manually and individually (i.e. clamping/unclamping each individual column). In the next generation of tests, we run not one column individually, but 96 at one time using commercially available filter/column plates in a 96-well format (96 columns in an 8 x 12 array). We tried a few different methods for stopping the flow from the well-plates for loading fluids or taking data at time points, with mixed results.


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Our described invention (Figure 1), a multi-well column manifold for liquid or gas chromatography, is to be applied to the...