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MICROORGANISMS AND METHODS FOR THE BIOSYNTHESIS OF ADIPATE, HEXAMETHYLENEDIAMINE AND 6-AMINOCAPROIC ACID

IP.com Disclosure Number: IPCOM000240369D
Publication Date: 2015-Jan-27
Document File: 247 page(s) / 3M

Publishing Venue

The IP.com Prior Art Database

Abstract

The invention provides a non-naturally occurring microbial organism having a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The invention additionally provides a method for producing 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid. The method can include culturing a 6-aminocaproic acid, caprolactam or hexametheylenediamine producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid.

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MICROORGANISMS AND METHODS FOR THE BIOSYNTHESIS OF ADIPATE, HEXAMETHYLENEDIAMINE AND 6-AMINOCAPROIC ACID

     Compared to previous publications this document (including the attached Supplementary File with Tabs A-N) includes additional gene classes and candidates for transformations catalyzed by enoyl-CoA reductases, thiolases, aminotransferases and amino acid dehydrogenases for pathways to make Nylon intermediates described in this document. The attached Supplementary File, an Excel document, contains several tabs (sheets) describing the additional gene classes and candidates for the transformations described herein. Sequences can be accessed form public sources, e.g. KEGG, UniProt, using the gene designations provided in the Supplementary File. Figures are at the end of this document.

     Tabs A-E of the Supplementary File provide additional enoyl-CoA reductases of EC classes 1.3.1 and 1.3.8 useful as a 5-carboxy, 2-pentenoyl-CoA reductase in Figures 2 and 9, Step D, Figure 10, Step D, Figure 11, Step N, Figure 12, and Step E, Figure 20.

     Tabs F-J of the Supplementary File provide additional aminotransferases and amino acid dehydrogenases of EC classes 1.4.1 and 2.6.1 useful as a transaminase and/or 6-aminocaproate dehydrogenase in Figure 8, Steps O, P, V, W in Figure 10, Steps O an P in Figure 11, Steps E, H and J in Figure 12, Steps C and G in Figure 13, Step in Figure 20, Steps D, E, J, Q, Z and AB in Figure 21, Steps D and G in Figure 22, Steps C and G in Figure 24 and Steps D, J, K and L in Figure 26.

     Tabs K-N of the Supplementary File provide additional thiolases of EC class 2.3.1 useful as a succinyl-CoA:acetyl- CoA transferase/beta-ketothiolases in Figures 2 and 3, Step A, Figure 10, Step B, Figure 20 and Step A, Figure 21.

ABSTRACT

     The invention provides a non-naturally occurring microbial organism having a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The invention additionally provides a method for producing 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid. The method can include culturing a 6-aminocaproic acid, caprolactam or hexametheylenediamine producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid.

BACKGROUND OF THE INVENTION

The present invention relates generally to biosynthetic processes, and more specifically to organisms having adipate, hexamethylenediamine, 6-aminocaproic acid and caprolactam biosynthetic capability.

Adipic acid,...