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Trap-door Tool for Controlling Flow in Multi-Well Chromatography and Use of Apparatus

IP.com Disclosure Number: IPCOM000241165D
Publication Date: 2015-Mar-31
Document File: 4 page(s) / 218K

Publishing Venue

The IP.com Prior Art Database

Abstract

A device and method for multi-well chromatography fluid flow control is described for gravitational, vacuum, and pressurized assays. Fluids can be flowed through a porous media and selectively collected at various time points, which cannot currently be accomplished with conventional vacuum filtration methods available for multi-well chromatography separations. The equipment described also successfully abates sealing and leak issues involved with conventional methods of sealing multi-well chromatography or filter-style plates, such as sealing mats, and provides a method for control of flow and flow rates for the plate without increasing backpressure from the outlet of the columns. The modifiable “trap door” manifold design is useful in a variety of fluid chromatography applications in areas such as oil and gas chemistry, solid phase extractions, chemical engineering, biotechnology, molecular biology, microbiology, pharmaceutical development and academic research.

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Trap-door Tool for Controlling Flow in Multi-Well Chromatography and Use of Apparatus

Abstract

A device and method for multi-well chromatography fluid flow control is described for gravitational, vacuum, and pressurized assays. Fluids can be flowed through a porous media and selectively collected at various time points, which cannot currently be accomplished with conventional vacuum filtration methods available for multi-well chromatography separations. The equipment described also successfully abates sealing and leak issues involved with conventional methods of sealing multi-well chromatography or filter-style plates, such as sealing mats, and provides a method for control of flow and flow rates for the plate without increasing backpressure from the outlet of the columns. The modifiable "trap door" manifold design is useful in a variety of fluid chromatography applications in areas such as oil and gas chemistry, solid phase extractions, chemical engineering, biotechnology, molecular biology, microbiology, pharmaceutical development and academic research.

Introduction

Currently, silicone-PTFE sealing mats are used to control the flows of fluids in the individual columns of high throughput column chromatography tests. Alternatively, all fluid flows in multi- well column chromatography applications are expedited through the use of vacuum pressure. Fluid in this paper is described as any substance that obeys fluid mechanics or dynamics principles, or continually deforms or flows under an applied shear stress.

In chromatography assays, the ability to stop, restart or otherwise control the flow of fluids is necessary for two purposes: 1) to prohibit the flow of fluids during column loading or incubation steps and 2) to temporarily stop the flow of fluids in order to take displacement measurements or fraction collections. Unfortunately, the current method of controlling flow allows leaks (resulting in errors, lost time and waste of materials), pressure buildup and column resin (or other solid material) disruption from air pressed upward into the columns from the use of sealing mats, and requires pulling or pressing actions which cause wear and tear on the sealing mats themselves. Vacuum filtration is considered an "all-or-none" option, with very little ability to fine-tune the flow of fluids evenly across the columns of the plate, and is inappropriate for assays in which flow rate studies are desired. The purpose of the described invention is to facilitate the control

Movement of trap-door plate

Figure 1. Top view of manifold embodiment. In this

configuration for a 96- well format, the manifold plate stack (purple and green pieces) is secured by a bolted compression support (orange). Handles can be placed on either side of the trap-door plate (green) or an all sides for user-friendliness. The trap door plate will slide lengthwise as allowed by the larger bolt-holes in the center which anchor the trap-door plate. The purple

plate will b...