ASSAY AND METHOD FOR TESTING
Publication Date: 2015-Aug-07
The IP.com Prior Art Database
The present invention concerns an assay for testing the presence of a fluorophore, a method for testing the precence of a fluorophore and an item comprising a flurophore.
ASSAY AND METHOD FOR TESTING
Reference to a Sequence Listing
This application contains a Sequence Listing in computer readable form, which is incorporated herein by reference.
Field of the Invention
The present invention concerns an assay and a method for testing the presence of a fluorophore on a first and/or a second surface. The invention further concerns an item with a surface comprising the composition and a composition comprising a fluorophore.
Description of the Related Art
A fluorophore absorbs light energy of a specific wavelength and re-emits light at a longer wavelength. The wavelengths of the absorbed light, energy transfer efficiency, and time before emission depend on both the fluorophore structure and its chemical environment, as the molecule in its excited state interacts with surrounding molecules. Wavelengths of maximum absorption (≈ excitation) and maximum emission (for example, Absorption/Emission = 485 nm/517 nm) are the typical terms used to refer to a given fluorophore, but the whole spectrum may be important to consider.
Excitation energies range from ultraviolet through the visible spectrum, and emission energies may continue from visible light into the near infrared region.
Wash performance has traditionally been addressed by use of remission values where an item after wash is submitted to light at 460 nm and the remission of light is measured and can be compared to the remission before wash as described in WO 2002/099091. However for some stains it is not possible to see the stain by human eye after wash. The item may appear clean after wash, even though the stain or residuals of the stain may still be present on the item. It is therefore very difficult to detect if an item is completely clean or if a part of the stain is still present after wash.
Summary of the Invention
The present invention concerns an assay for testing the presence of a fluorophore on a first and/or a second surface comprising the following steps:
a. Preparing a composition comprising a fluorophore having an absorbance wavelength and an emission wavelength,
b. applying the composition to the first surface,
c. submitting the first surface and/or the second surface to a liquid composition,
d. optionally rinsing and/or drying the first surface and/or the second surface,
e. exposing the first surface and/or the second surface to light having a wavelength within +/- 30 nm of the absorbance wavelength of the fluorophore, and
f. detecting the light emitted from the fluorophore present on the first surface and/or the second surface.
The invention further concerns a method for testing the presence of a fluorophore on a first and/or a second surface comprising the following steps:
- Preparing a composition comprising a fluorophore having an absorbance wavelength and an emission wavelength,
- applying t...