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Uronate Dehydrogenase for the Production of Glucaric Acid based Corrosion Inhibitors

IP.com Disclosure Number: IPCOM000243709D
Publication Date: 2015-Oct-14
Document File: 4 page(s) / 19K

Publishing Venue

The IP.com Prior Art Database

Related People

Baker Hughes Incorporated: OWNER [+4]

Abstract

Most industrial cooling towers utilize orthophosphate polyphosphates or other phosphorous containing water treatment programs to mitigate corrosion problems However the use of such corrosion inhibitors is becoming the object of federal and local regulations because of phosphorous contamination of surface water To address this need it has been found that salts of glucaric acid and or galactaric acid are efficient carbon steel corrosion inhibitors for cooling water systems These are non phosphorous organic materials that can be easily fine tuned to cater different cooling water conditions including systems where the phosphorous based corrosion inhibitors fail The ability to make glucaric acid glucaric acid salts and galactaric acid economically will not only help capture a wider market of new and robust cooling water corrosion inhibitors but more importantly helps in the development of a more environmentally safe solutions for water treatment programs

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Uronate Dehydrogenase for the Production of Glucaric Acid-based Corrosion Inhibitors

Most industrial cooling towers utilize orthophosphate, polyphosphates or other phosphorous-containing water treatment programs to mitigate corrosion problems. However, the use of such corrosion inhibitors is becoming the object of federal and local regulations because of phosphorous contamination of surface water. To address this need, it has been found that salts of glucaric acid and/or galactaric acid are efficient carbon steel corrosion inhibitors for cooling water systems. These are non-phosphorous organic materials that can be easily fine-tuned to cater different cooling water conditions including systems where the phosphorous-based corrosion inhibitors fail. The ability to make glucaric acid, glucaric acid salts, and galactaric acid economically will not only help capture a wider market of new and robust cooling water corrosion inhibitors but more importantly helps in the development of a more environmentally-safe solutions for water treatment programs.

Current technology involves producing the glucaric acid either synthetically or via fermentation processes where a wild-type or genetically modified organism produces glucaric acid via a metabolic pathway, often starting from glucose. One additional method to produce glucaric acid is through the uronate dehydrogenase enzyme catalyzed pathway.  This technology involves the use of the purified or crude enzyme, urinate dehydrogenase (synonym: uronate:NAD-oxidoreductase, uronic acid dehydrogenase, EC 1.1.1.203) for the production of glucaric acid from galacturonate or glucoronate in a reaction vessel or in situ.  This method does not involve the full metabolic pathway that other methods use and is used for the sole purpose of producing corrosion inhibitor chemicals.

Uronate dehydrogenase is a homohexamer and utilizes the NADH cofactor to produce glucaric acid from galacturonate or glucoronate.  This enzyme includes a Rossmann fold for the binding of NADH within the active site and has a molecular weight of approximately 59,000 Da as determined by gel filtration and ultracentrufugation. Although this enzyme can be found in many biological sources, the preferred material for this work most likely will center on the enzyme from Agrobacterium tumefaciens, Agrobacterium tumefaciens strain C58, Pseudomonas putida KT2440, Pseudomonas syri...