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ClipCulture or Clipture A type of convenient multi well plates for cellular assays

IP.com Disclosure Number: IPCOM000244185D
Publication Date: 2015-Nov-20
Document File: 2 page(s) / 138K

Publishing Venue

The IP.com Prior Art Database

Abstract

Multi well plates are commonly used for cellular assays world widely However it is very time consuming to re plate feed cells on multi well plates The well well variation and edge effect also limited the use of traditional multi well plates for high content assays Furthermore certain cell types such as primary human hepatocytes don t attach well on some traditional multi well plates such as 96 well plates and 384 well plates Here we propose a type of convenient multi well plates to solve these problems

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ClipCulture or Clipture: A type of convenient multi-well plates for cellular assays

Introduction:

Multi-well plates are commonly used for cellular assays world widely. However, it is very time-consuming to re-plate, feed cells on multi-well plates. The well-well variation and edge-effect also limited the use of traditional multi-well plates for high content assays. Furthermore, certain cell types such as primary human hepatocytes don’t attach well on some traditional multi-well plates such as 96 well plates and 384 well plates. Here, we propose a type of convenient multi-well plates to solve these problems.

 Design:

The whole plate contains four different layers as shown and described in Figure 1. These layers are only description for internal use and the product should be separately packed as two sterilized parts: layer 2 and layer 1&3&4 together. Layer 3 and 4 should be glued together in the package.

Figure 1: The diagram of the plate from side. Layer 1 is the cover of the plates. This cover should be able to achieve a complete seal; Layer 2 is the well insert. The insert can be single well format or co-culture format which will be described later; Layer 3 is the silicon hydrophobic non-culture layer with shallow holes for cells to reach the culture layer; Layer 4 is the cell culture suitable layer with all the necessary coating for cell growth.

The view of part3/4 is shown in Figure 2. The culture area should be slightly lower (1mm lower) than the hydrophobic non-culture area. The advantage of this design is that the cells can attach on the culture area only but medium can be exchanged freely across different wells. As the lid of this type of plates should achieve complete seal, it will be required to use gas permeable plastic for layer 4 to allow CO2 and O2 exchange. We use this design to minimize the edge effect which is a common problem of most commercially available multiwell plates.

Figure 2: The top view of part 3/4. The culture ...