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BACILLUS STRAINS IMPROVING HEALTH AND PERFORMANCE OF PRODUCTION ANIMALS

IP.com Disclosure Number: IPCOM000245129D
Publication Date: 2016-Feb-11

Publishing Venue

The IP.com Prior Art Database

Related People

Marianne Thorup Cohn: INVENTOR [+6]

Abstract

The present invention relates to animal feed or animal feed additive comprising Bacillus strains which improve the health and performance of production animals. The invention further relates use of the Bacillus strains in animal feed and animal feed additives.

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Bacillus strains improving health and performance of production animals

Reference to a Deposit of Biological Material

     This application contains a reference to a deposit of biological material, which deposit is incorporated herein by reference.

Reference to sequence listing

     This application contains a Sequence Listing in computer readable form. The computer readable form is incorporated herein by reference.

Index to sequence listing:

SEQ ID NO: 1 is 16S rDNA of DSM 29869.

SEQ ID NO: 2 is 16S rDNA of DSM 29870.

SEQ ID NO: 3 is 16S rDNA of DSM 29871.

SEQ ID NO: 4 is 16S rDNA of DSM 29872.

SEQ ID NO: 5 is 16S rDNA of Bacillus vallismortis from AB021198.

SEQ ID NO: 6 is 16S rDNA of Bacillus subtilis from AJ276351.

SEQ ID NO: 7 is 16S rDNA of Bacillus amyloliquefaciens from AB255669. SEQ ID NO: 8 to SEQ ID NO: 13: PCR and sequencing primers.

BACKGROUND OF THE INVENTION

Field of the Invention

     The present invention relates to an animal feed or an animal feed additive comprising Bacillus strains which improve the health and performance of production animals. The invention further relates to use of the Bacillus strains in animal feed and animal feed 25

additives.

Background of the Invention

     Clostridium perfringens (C. perfringens) is a Gram-positive, rod-shaped, anaerobic, spore-forming bacterium of the genus Clostridium. C. perfringens is widely present in nature and can be found as a component of decaying vegetation, marine sediment, the intestinal tract of humans and other vertebrates, insects, and soil.

     Infections due to C. perfringens show evidence of tissue necrosis, bacteremia, emphysematous cholecystitis, and gas gangrene, which is also known as clostridial myonecrosis. C. perfringens can also result in polymicrobial anaerobic infections. 35

     The incidence of Clostridium perfringens-associated necrotic enteritis in poultry has increased in countries that stopped using antibiotic growth promoters. Necrotic enteritis is an enterotoxemic disease that results in significant economic losses in the poultry industry.

     There is a need to develop tools and strategies for the prevention and control of C. perfringens in mono-gastric animals such as poultry. Whilst the vaccination of animals has been suggested, there are challenges associated with vaccinating thousands of animals. Thus discovering a solution which could be administered as an additive in an animal feed would be advantageous.

     It is thus an object of the invention to provide solutions which prevents and/or controls
C. perfringens in mono-gastric animals such as poultry by use of an animal feed comprising one or more bacteria with activity against Clostridium perfringens.

     A further object of the invention is to provide a solution that does not present a risk for the health of the animal. The solution to this problem is use of non-hemolytic Bacillus strains with activity against Clostridium perfringens.

A challenge of delivering Bacillus spp. in feed is the common use of antibiotics...