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De-Novo Signal Concepts in FLASH chromatography

IP.com Disclosure Number: IPCOM000245164D
Publication Date: 2016-Feb-16
Document File: 2 page(s) / 159K

Publishing Venue

The IP.com Prior Art Database

Abstract

Flash chromatography is a form of liquid chromatography (LC), which is widely used for purification of low molecular weight organic compounds and products of organic synthetic reactions. In Flash chromatography, typically, the mobile phase is first admitted into a space above a bed of stationary phase, and then pushed through the bed with gas pressure. Once the space is free again, the sample is admitted, and it too is pushed into the bed, displacing the earlier charge of neat mobile phase. Then in a third step, a second charge of mobile phase moves the solution through the bed, causing fractionation of the sample. Fractions resulting from the Flash chromatography column may be collected, and are commonly detected by mass spectrometry and/or UV detection.

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De-Novo Signal Concepts in FLASH chromatography

Background

Flash chromatography is a form of liquid chromatography (LC), which is widely used for

purification of low molecular weight organic compounds and products of organic synthetic

reactions. In Flash chromatography, typically, the mobile phase is first admitted into a space

above a bed of stationary phase, and then pushed through the bed with gas pressure. Once the

space is free again, the sample is admitted, and it too is pushed into the bed, displacing the

earlier charge of neat mobile phase. Then in a third step, a second charge of mobile phase

moves the solution through the bed, causing fractionation of the sample.

Fractions resulting from the Flash chromatography column may be collected, and are

commonly detected by mass spectrometry and/or UV detection.

Current Technology

Systems for flash chromatography have been available for some of years, such as Isolera™

(http://www.biotage.com) and the CombiFlash™ purification systems marketed by Teledyne

ISCO. More recently, other commercial systems have been introduced which directly couple

flash chromatography to mass spectrometry (MS) to provide more automated systems for

purification. An example of such a system is the Isolera ™ Dalton (http://www.biotage.com).

Methods have been proposed in the art for collecting 'composite' signals from detectors when analyzing the output from chromatography columns, such as flash chromatography columns. For example, a method of analyzing a sample may comprises the steps of generating such a 'composite' signal from two or more detectors in a liquid chromatography system, the composite signal comprising a detection response component from each detector; and collecting a new sample fraction in a fraction collector in response to a change in the composite signal. The 'composite' signal may comprise (i) a detection response component from at least one optical absorbance detector, such as a UV detector, and (ii) a detection response component from at least one evaporative particle detector. Alternatively, the 'composite' signal may comprise (i) a detection response component comprising two or more detector responses from an optical absorbance detector, such as a UV detector, at two or more specific optical wavelengths and (ii) a detection response component...