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The use of enzymes in detergents

IP.com Disclosure Number: IPCOM000246001D
Publication Date: 2016-Apr-25

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The IP.com Prior Art Database

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Page 01 of 16

Detergent research disclosure template

The use of enzymes in detergents

The application of enzymes in granular or liquid detergent compositions for use in

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cleaning processes such as laundry, hard surface cleaning, including manual and automatic

dish wash (ADW) is now common.

     Soiling comes in many forms and includes proteins, starches and lipids. Using detergents in water at high temperatures and with vigorous mixing, it is possible to remove most types of soils but the cost of heating the water is high and lengthy mixing or beating will shorten

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the life of clothing and other materials. The use of enzymes allows lower temperatures to be employed and shorter periods of agitation are needed, often after a preliminary period of soaking. In general, enzyme detergents remove protein from clothes soiled with blood, milk, sweat, grass, etc. far more effectively than non-enzyme detergents.

At present proteases and alpha-amylases are commonly used in detergent 15

compositions.

Exemplary proteases known from detergent applications are shown in table 1.

Table 1. Proteases known from detergent applications
PR001 Protease from Bacillus clausii shown in SEQ ID NO:1
PR002 Variant of PR001 with the alteration M216S
PR003 Variant of PR001 with the alterations *36D+ N74D+ H118D+ G189E+ K229L PR004 Variant of PR001 with the alterations Y161A+ R164S+ A188P
PR005 Variant of PR001 with the alterations S97SE
PR006 Variant of PR001 with the alterations V66A+ S104A
PR007 Variant of PR001 with the alterations S9R+ A15T+ V66A+ N212D+ Q239R PR008 Variant of PR001 with the alterations S9R+ A15T+ G59E+ V66A+ A188P+

       V199I+ Q239R+ N255D
PR009 Variant of PR001 with the alterations S97AD
PR010 Protease from Bacillus licheniformis shown in SEQ ID NO:2 PR011 Protease from Bacillus halodurans shown in SEQ ID NO:3 PR012 Protease from Bacillus amyloliquefaciens shown in SEQ ID NO:4 20

Exemplary alpha-amylases known from detergent applications are shown in table 2.

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Page 02 of 16

Detergent research disclosure template

Table 2. Alpha-amylases known from detergent applications.

AM001 Variant of SEQ ID NO: 12 in WO 2001/066712 with the alterations D183*+ G184*+

        R118K+ N195F+ R320K+ R458K
AM002 Variant of SEQ ID NO: 12 in WO 2001/066712 with the alterations M9L+ R118K+

G149A+ G182T+ G186A+ Del(D183-G184) + N195F+ M202L+ T257I+ Y295F+ N299Y+ R320K+ M323T+ A339S+ E345R+ R458K
AM003 Variant of SEQ ID NO: 2 in WO 1996/023873 with the alterations D183*+ G184* AM004 Variant of SEQ ID NO: 2 in WO 1996/023873 with the alterations W140Y+ D183*+

        G184*N195F+ V206Y+ Y243F+ E260G+ G304R+ G476K
AM005 Variant of SEQ ID NO: 2 in WO 1997/041213 with the alterations H156Y+ A181T+

        N190F+ A209V+ Q264S
AM006 Variant of SEQ ID NO: 2 in WO 1997/041213 with the alterations G48A+ T49I+

G107A+ H156Y+ A181T+ I201F+ A209V+ Q264S

     Proteases and alpha-amylases can be applied together with additive or even synergistic effect in detergent compositions for use in cleaning processes such as laundry, hard surface...