Enzymatic alginate extraction
Publication Date: 2016-Aug-17
The IP.com Prior Art Database
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Enzymatic Alginate Extraction Enhancement Technique
Below is described a highly effective method for extracting alginate from seaweed algae (such as Lessonia Nigrescens), which improves the extraction rate and viscosity of sodium alginate. The method uses an enzymatic hydrolysis of the seaweed with a cellulase preparation, where the cellulase content is at least 60% of CBHI and CBHII cellulases. The process steps are as follows:
1. The dry algae (for example Lessonia Nigrescens) are soaked in tap water and formaldehyde at room temperature for 2-12 hours.
2. After draining, fresh tap water is added and the mixture is optionally soaked again for 12-24 h.
3. The algae are washed twice with tap water to remove excessive formaldehyde.
4. The algae are ground to a particle size of about 5-20mm.
5. Water is added to the ground algae particles, the temperature is increased to 40-60°C, pH is adjusted to slightly acidic, and a cellulase preparation is added. At least 60% of the cellulase(s) in the cellulase preparation are CBHI and/or CBHII cellulases. The mixture is stirred for 1-4 hours.
6. After enzymatic hydrolysis of the mixture, the pH is adjusted to alkaline by addition of, for example, sodium carbonate or a similar reagent that increases pH to alkaline. The mixture is stirred for 1-10 hours.
7. After alkali treatment, the mixture is diluted with water and filtrated to remove solid remnants of the algae.
8. A flocculant (normally chitin) is added to the filtrated...