Browse Prior Art Database

Split Detection System for RNA, with Applications in Research and Molecular Diagnostics

IP.com Disclosure Number: IPCOM000248023D
Publication Date: 2016-Oct-19
Document File: 5 page(s) / 99K

Publishing Venue

The IP.com Prior Art Database

Abstract

Ribonucleic acid (RNA) is a multifunctional type of molecule, playing critical roles in protein biosynthesis and regulation. In recent years, suppression of protein translation by so-called microRNAs (miRNAs) came into the focus of research, especially because deregulation of this process has been shown to play a role in malignant transformation. Furthermore, RNA molecules circulating in the blood have been revealed as a novel class of markers for diagnosis of cancers. Moreover, genetic information of some pathogens is stored as RNA, allowing their sensitive detection using nucleic acid amplification techniques. Furthermore, the emerging use of microRNA (miRNA) and circulating RNA profiles complementing the broad spectrum of RNA diagnosis. Hence, miRNAs have emerged as a valuable tool for diagnosis and clinical treatment due to several distinct features. One of these characteristics is that cancer cells exhibit different miRNA profiles. This enables the discrimination between normal and cancer tissues, making miRNA expression profiles useful for molecular diagnosis of tumours. Changes in miRNA expression profiles have been reported for a number of different cancers including colorectal neoplasia, paediatric Burkitt lymphoma, small lung carcinoma, breast cancer, papillary thyroid cancer and B-cell lymphoma. Furthermore, miRNA expression profiles can not only be used to separate different cancer types, but also to classify cancer differentiation states and cluster sample groups according to their embryonic lineage. The present invention describes a novel method for detection of wide range of RNA molecules for analytical and diagnostic purposes. The method combines isothermal reverse transcription (RT) of target RNA, transcription with viral RNA polymerases (T7, T3 or SP6) and fluorescent based real-time detection of the newly synthetized RNA product. The approach can be used for detection of any type of RNA including viral RNA, mRNA, miRNA, and potentially, RNA sensors, monitoring events within live cells, RNA-protein interactions, mRNA trafficking, RNAase activity, fluorescence in situ hybridisation, and reporter gene assays. The invention involves a coupled detection technology involving RNA aptamers and has not been previously reported elsewhere. The invention described here is applicable to nucleic acid research, genetics, molecular diagnostics and human identification processes

This text was extracted from a PDF file.
This is the abbreviated version, containing approximately 33% of the total text.

Page 01 of 5

Split Detection System for RNA, with Applications in Research and Molecular Diagnostics

Summary

Ribonucleic acid (RNA) is a multifunctional type of molecule, playing critical roles in protein biosynthesis and regulation. In recent years, suppression of protein translation by so-called microRNAs (miRNAs) came into the focus of research, especially because deregulation of this process has been shown to play a role in malignant transformation. Furthermore, RNA molecules circulating in the blood have been revealed as a novel class of markers for diagnosis of cancers. Moreover, genetic information of some pathogens is stored as RNA, allowing their sensitive detection using nucleic acid amplification techniques. Furthermore, the emerging use of microRNA (miRNA) and circulating RNA profiles complementing the broad spectrum of RNA diagnosis.

Hence, miRNAs have emerged as a valuable tool for diagnosis and clinical treatment due to several distinct features. One of these characteristics is that cancer cells exhibit different miRNA profiles. This enables the discrimination between normal and cancer tissues, making miRNA expression profiles useful for molecular diagnosis of tumours. Changes in miRNA expression profiles have been reported for a number of different cancers including colorectal neoplasia, paediatric Burkitt lymphoma, small lung carcinoma, breast cancer, papillary thyroid cancer and B-cell lymphoma. Furthermore, miRNA expression profiles can not only be used to separate different cancer types, but also to classify cancer differentiation states and cluster sample groups according to their embryonic lineage.

The present invention describes a novel method for detection of wide range of RNA molecules for analytical and diagnostic purposes. The method combines isothermal reverse transcription (RT) of target RNA, transcription with viral RNA polymerases (T7, T3 or SP6) and fluorescent based real-time detection of the newly synthetized RNA product. The approach can be used for detection of any type of RNA including viral RNA, mRNA, miRNA, and potentially, RNA sensors, monitoring events within live cells, RNA-protein interactions, mRNA trafficking, RNAase activity, fluorescence in situ hybridisation, and reporter gene assays.

The invention involves a coupled detection technology involving RNA aptamers and has not been previously reported elsewhere.

The invention described here is applicable to nucleic acid research, genetics, molecular diagnostics and human identification processes.

Introduction

The detection and quantification of ribonucleic acid (RNA) molecules has emerged as an invaluable tool for medical diagnosis and for addressing basic research issues. This process covers the analysis of pathogenic RNA, messenger RNA (mRNA), circulating RNA, and miRNA molecules. The latter species has drawn increasing


Page 02 of 5

attention because deregulation of miRNAs has been shown to be accompanied with malignant transformation, such as canc...