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Alternative Generation of Antimicrobial sample collection cards for DNA, RNA and proteins

IP.com Disclosure Number: IPCOM000248257D
Publication Date: 2016-Nov-11
Document File: 6 page(s) / 94K

Publishing Venue

The IP.com Prior Art Database

Abstract

A number of selected antimicrobials and possible methods to covalently attach or impregnate them to solid support membrane surface have been presented. The appropriate candidate antimicrobial selected for attaching to the membrane surface should be inexpensive, exhibit prolonged stability and be non-toxic to users. Candidates reagents should exhibit the potential to kill a broad spectrum of pathogens and should not negatively affect the biological sample applied to the coated sample collection card. In addition antimicrobial sample card collection cards such not promote any allergic reaction to the end user if inappropriate contact occurs. It may also be advantageous to attached naturally-occurring antimicrobials onto the membranes to develop ecology friendly sample collection cards; such cards may also be convenient in the face of expanding Environmental, Health and Safety regulations. Overall, the ideas described in this invention will lead to the development of new sample collection cards that exhibit enhance cost effective antimicrobial properties, whilst still retaining the ability to facilitate the prolonged storage of biomolecules such as DNA, RNA etc. The combination of a protease with allicin and/or oleuropein can potentially generate sample collection cards that exhibit a broad antimicrobial spectrum and are therefore effective at killing a broad range of microbes. The use of a nitrocellulose matrix over a one consisting of un-modified cellulose has the advantage of increased efficiency for covalently attaching biomolecules especially antibiotics, proteins etc.

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Alternative Generation of Antimicrobial sample collection cards for DNA, RNA and proteins

Introduction

Collecting and archiving biological samples for subsequent laboratory tests is an essential step that influences the quality of results derived from downstream molecular biological and genetic analyses. For achieving optimal conditions, the collected biological samples, typically DNA, RNA (and protein), require optimal collection, handling and storage conditions. Therefore, the collected samples should be kept in an environment that stabilises analytes of interest and is free of factors that could introduce any deterioration or degradation to the sample.

Currently Whatman FTA, FTA Elute and Protein Saver 903 cards (GE Healthcare) and NucleoSave card (Macherey Nagel) are cellulose-based commercially-available products for sample collection, transportation and storage. FTA and FTA Elute exhibit strong anti-microbial properties, which is derived from the proprietary nucleic acid stabilising chemicals impregnated on the paper. In contrast Protein Saver 903 and NucleoSave cards lack any DNA stabilising or antimicrobial activity.

To generate optimal downstream sample analyses the inactivation of bacterial growth or the associated enzymatic activities, is essential to prevent sample or analyte degradation. Bacterial growth and the associated enzymatic activities could potentially have detrimental effects on the sample quality and this might result in the generation of non-optimal data in, for example; diagnostic tests. Therefore it is important to minimize the potential contamination and subsequent growth of bacterial, fungal, nucleic acid etc. on sample collection cards.

This invention describes several different approaches that all facilitate the generation of sample collection cards that exhibit antimicrobial activity using reagents different to those currently used for commercially-available sample collection cards. These in combination with existing membrane technology offer solid supports that deliver low levels of extractable material, high temperature stability, mechanical strength and flexibility, thus producing promising candidates for generating the next generation of antimicrobial sample collection cards. For example an extremely promising alternative is to produce solid supports that combine the natural antimicrobial reagents allicin and oleuropein with a protease.

In the following paragraphs different antimicrobial components and combination thereof are discussed, these include; antimicrobials from plant, antimicrobial enzymes, cationic polymers and ionophores. These are given as way of example and should not be considered as limiting, as different combination of components will still be able to deliver the required antimicrobial activity. Inherent in the description is the enabling feature of combining, these anti-microbial chemicals by coating, impregnating, covalently attaching etc., them to solid support biolog...