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The use of enzymes in detergents

IP.com Disclosure Number: IPCOM000248840D
Publication Date: 2017-Jan-17

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The use of enzymes in detergents

The use of enzymes in granular or liquid detergent compositions for use in cleaning processes such as hard

surface cleaning, dish wash, manual dish wash, automatic dish wash (ADW) and laundry (hand or machine

wash) is now common.

Dirt comes in many forms and includes proteins, starches and lipids. Using detergents in water at high

temperatures and with vigorous mixing, it is possible to remove most types of dirt, however the cost of

heating the water is high and lengthy mixing/agitation/beating will shorten the life of clothing and other

materials. The use of enzymes allows lower temperatures to be employed and shorter periods of agitation are

needed, often after a preliminary period of soaking. In general, enzyme detergents remove protein from

clothes soiled with blood, milk, sweat, grass, etc. far more effectively than non-enzyme detergents.

At present, proteases and alpha-amylases are the most commonly used enzymes in detergent compositions,

but other enzymes such as lipases, carbohydrases and cutinases are also used. Exemplary alpha-amylases

known from detergent applications are shown in table 1. Exemplary proteases known from detergent

applications are shown in table 2. Exemplary lipases known from detergent applications are shown in table 3.

Exemplary carbohydrases known from detergent applications are shown in table 4. Exemplary cutinases

known from detergent applications are shown in table 5.

Code Table 1: Exemplary alpha-amylases for use in a detergent composition

AM001 Variant of SEQ ID NO 12 in WO 2001/066712 with the alterations D183*+ G184*+ R118K+

N195F+ R320K+ R458K

AM002 Variant of SEQ ID NO 12 in WO 2001/066712 with the alterations M9L+ R118K+ G149A+

G182T+ G186A+ D183* + G184* + N195F+ M202L+ T257I+ Y295F+ N299Y+ R320K+ M323T+

A339S+ E345R+ R458K

AM003 Variant of SEQ ID NO 2 in WO 1996/023873 with the alterations D183*+ G184*

AM004 Variant of SEQ ID NO 2 in WO 1996/023873 with the alterations W140Y+

D183*+G184*+N195F+V206Y+Y243F+E260G+G304R+G476K

AM005 Variant of SEQ ID NO 2 in WO 1997/041213 with the alterations H156Y+ A181T+ N190F+

A209V+ Q264S

AM006 Variant of SEQ ID NO 2 in WO 1997/041213 with the alterations G48A+ T49I+ G107A+ H156Y+

A181T+ I201F+ A209V+ Q264S

AM007 Variant of SEQ ID NO 1 with the alteration M202L

AM008 Variant of SEQ ID NO 2 with the alterations R180*+ S181* + S243Q + G475K + CBM* (domain)

AM009 Variant of SEQ ID NO 3 with the alterations R178* + G179* + E187P + I203Y + R458N + T459S +

D460T + G476K

AM010 Amylase of SEQ ID NO 4

AM011 Amylase of SEQ ID NO 5

AM012 Amylase of SEQ ID NO 6

AM013 Amylase of SEQ ID NO 7

2

AM014 Amylase of SEQ ID NO 8

AM015 Amylase of SEQ ID NO 9

AM016 Amylase of SEQ ID NO 10

Code Table 2: Exemplary proteases for use in a detergent composition

PR001 Protease comprising the amino acid sequence shown in SEQ ID NO:11

PR002 Variant of PR001 with the alteration M216S

PR003 Variant of PR001 with the alterations *36D+N74D+H118D+G189E+K229L

PR004 Var...