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96 Well Ready to Spot, Ready to Go, FTA Plate (RTS_RTG_FTA)

IP.com Disclosure Number: IPCOM000249500D
Publication Date: 2017-Mar-01
Document File: 5 page(s) / 182K

Publishing Venue

The IP.com Prior Art Database

Abstract

The following idea relates to FTA cards, but could also apply to 903 cards. Also, with some minor changes in workflow, this could apply to FTA Elute cards.

The current GE Healthcare FTA technology sample process workflow requires biological samples to first be applied to the FTA paper, dried and then a small disc is punched out. The disc can then be washed and placed in a 'punch in' PCR reaction or the punch can be directly added to the PCR reagents and amplified. The following concepts have been devised to simplify the FTA workflow process and reduce sample process times.

Concept 1. Provide FTA paper pre-punched in a 96-well format device. Manufacture FTA in pre-punched format, customers can then spot their samples directly, negating the need to spot and punch. Providing a convenient 96 well format-holding device would aid sample application, allow drying and storage. Refer to Figures 1 and 2 for details.

Concept 2. Invent a 96 well plate format that combines the use of the pre-cut FTA punches (above) with the GE Healthcare Ready-To-Go (RTG) reagents for PCR. The two device formats are combined, offering RTG reagents in an integral format with the pre-punched FTA punches or separately to offer more flexibility for sample processing. The FTA can be used to lyse cells, inactivate proteins and stabilise DNA in the biological sample. The RTG components can then be used for PCR amplification. Refer to Figures 3, 4 and 5 for details.

This invention could also be applied to other processes that use downstream enzymatic reactions, such as STR profiling, whole genome amplification and RT-PCR. Other sample collection cards / paper formats (e.g., neonatal screening cards 903, FTA elute, RSM) could be used.

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251096

96 Well Ready to Spot, Ready to Go, FTA Plate (RTS_RTG_FTA)

FTA technology (a Trade Mark of GE Healthcare - GEHC) The GEHC FTA cards and FTA Elute cards are intended for convenient sample collection and purification. Following simple purification steps, DNA remains bound to the matrix of FTA cards, which can then be used directly for downstream applications. With FTA Elute cards, DNA will be eluted into water after purification. The eluted DNA can be used for quantitative DNA analysis. FTA cards and FTA Elute cards utilize patented Whatman technology that simplifies collection, transportation, and storage of biological samples and processing of nucleic acids at room temperature. The sample is simply applied to the FTA card or FTA Elute card, which contains chemicals that lyse cells, denature proteins, and protect nucleic acids from nucleases. The released nucleic acids are entrapped and preserved in the fibers of the matrix.

Ready-To-Go (RTG) technology The GEHC Ready-To-Go (RTG) technology consists of pre-formulated and pre- dispensed, freeze-dried PCR reagent mixtures in a bead format for robust and reproducible performance in standard PCR amplifications. The RTG technology has also been applied to GenomiPhi (Whole genome amplification), Hot start and RT-PCR applications. The advantages are as follows:-

 Save time: simply add template DNA solution and primers, and cycle.  More reproducible results: less risk of pipetting errors and contamination.  High-purity reagents: robust performance and the lowest possible levels of

contaminating DNA.  Long-term ambient-temperature stable: no freezer space required; less energy

consumption for shipping and storage.  Verified for real-time PCR.  For highly robust and reproducible performance in standard PCR applications.

SUMMARY OF IDEA

The following idea relates to FTA cards, but could also apply to 903 cards. Also, with some minor changes in workflow, this could apply to FTA Elute cards.

The current GE Healthcare FTA technology sample process workflow requires biological samples to first be applied to the FTA paper, dried and then a small disc is punched out. The disc can then be washed and placed in a ‘punch in’ PCR reaction or the punch can be directly added to the PCR reagents and amplified. The following concepts have been devised to simplify the FTA workflow process and reduce sample process times.

Concept 1. Provide FTA paper pre-punched in a 96-well format device. Manufacture FTA in pre-punched format, customers can then spot their samples directly, negating the need to spot and punch. Providing a convenient 96 well format-holding device would aid sample application, allow drying and storage. Refer to Figures 1 and 2 for details.

251096

Concept 2. Invent a 96 well plate format that combines the use of the pre-cut FTA punches (above) with the GE Healthcare Ready-To-Go (RTG) reagents for PCR. The two device formats are combined, offering RTG reagents in an integral format with the pr...