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Food Grade Dye for Online Integrity test based on Electrostatic Interactions

IP.com Disclosure Number: IPCOM000249503D
Publication Date: 2017-Mar-01
Document File: 6 page(s) / 240K

Publishing Venue

The IP.com Prior Art Database

Related People

Dr. Raoudha HADDAD: INVENTOR [+3]

Abstract

This document discloses the use of a food grade dye having an electrostatic charge which is close to the isoelectric point of MS2 virus (3.9). This means that the MS2 virus and the food grade dye have the same electrostatic charge in the same pH range. The food dye can be used to test online the effectiveness of a water filter containing Ahlstrom's Disruptor® media in removing biological contaminations while the filter is in use. The food grade dye successfully simulates the behavior of the Disruptor® media with MS2 virus and overcomes the limitations of other known methods of biological testing. The dye can also be used to check if the Disruptor® media is saturated and verify visually the effectiveness of cartridge design.

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Food Grade Dye for Online Integrity test

based on Electrostatic Interactions

March 1, 2017

Dr. Raoudha HADDAD

Ahlstrom Specialties

Product Development Center

Etablissement de Pont-Evêque

Z.I. de L’Abbaye

364 Impasse Louis Champin

F-38780 Pont-Evêque, France

© Ahlstrom Corporation 2017

1 Abstract

This document discloses the use of a food grade dye having an electrostatic

charge which is close to the isoelectric point of MS2 virus (3.9). This means

that the MS2 virus and the food grade dye have the same electrostatic charge

in the same pH range. The food dye can be used to test online the

effectiveness of a water filter containing Ahlstrom’s Disruptor® media in

removing biological contaminations while the filter is in use. The food grade

dye successfully simulates the behavior of the Disruptor® media with MS2

virus and overcomes the limitations of other known methods of biological

testing. The dye can also be used to check if the Disruptor® media is

saturated and verify visually the effectiveness of cartridge design.

2 Keywords

Erythrosin B, Food grade dye, Electrostatic interaction, Water filtration,

Integrity test, MS2 virus

3 Introduction

Ahlstrom’s Disruptor® media has a natural electrostatic positive charge that

maintains integrity in pH lower than 11.5. The filter media has an extremely

high surface area and a large number of active sites for submicron

contaminant removal by electro-adsorption. That is, the filter media has

enhanced removal capability for negatively charged submicron particles such

as viruses, bacteria, organic acids, endotoxins, etc.

It is not possible to test the sub-micron removal efficiency of Disruptor® using

methods typically used for nonwoven macro-filtration media. Conventional

tests like bubble point and pore size are not indicative of pathogen reduction

or submicron filtration efficiency of electro-adsorptive media. Currently, filters

are tested with samples containing MS2 virus to evaluate removal of

biological contamination. However, biological testing takes time and cannot be

carried out online while the filter is in use.

This document describes the insertion of a food-grade dye for online integrity

testing of filters containing Disruptor® media- the food grade dye successfully

simulates the MS2 virus and can be used to visually validate that the filter is

removing viral contaminants while it is being used.

4 Detailed Description

The MS2 virus has an isoelectric point of 3.9, which makes it negatively

charged at pH higher than 3.9. In order to simulate the electrostatic behavior

of the MS2 virus in the Disruptor® media, a contaminant with pKa close to the

isoelectric point of MS2 virus is required. Additionally, the contaminants used

for online testing have to be visible to the naked-eye and either food-contact

approved or an edible dye.

For example, the red food-dye Erythrosin B, which has a pKa of 4.1, can be

used to successfully test a Disruptor®-based filter or flat-sheet media. A

challenge solution of Er...