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Detergent compositions comprising lipase variants

IP.com Disclosure Number: IPCOM000006553D
Publication Date: 2002-Jan-14
Document File: 8 page(s) / 159K

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The IP.com Prior Art Database

Related Documents

WO 0060063: PATAPP

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Detergent compositions comprising lipase variants

This disclosure relates to detergent compositions comprising a lipase.


The lipase is a variant of the Humicola lanuginosa (Thermomyces lanuginosus) lipase with the substitutions T231R +N233R or another variant described in WO 0060063.

The lipase may be included in the detergent composition in the form of a non-dusting granulate, a stabilized liquid, or a protected enzyme as described in Research Disclosure No. 35944 published on 10 March 1994 by Novo Nordisk.

The addition of the lipase to the detergent may provide benefits such as stain removal, removal of fatty stains, whiteness maintenance, anti-redeposition or dinginess reduction.

The dosage of the lipase may correspond to a lipase activity in the wash solution of 100 LU/l, 500, 800, 1000, 2000, 3000, 3500, 4000, 6000 or 12000 LU/l detergent solution. The lipase dosage in the wash solution may be 0.02, 0.05, 0.1, 0.2, 0.3, 0.4, 1 or 2 mg/l as enzyme protein. The LU unit for lipase activity is defined in WO 9942566.

Detergent composition

The detergent composition may be in any convenient form, e.g. as powder, granules, paste, tablets, or liquid. A li­quid detergent may be aqueous, typically containing up to 70% water and 0-30% organic solvent, or non-aqueous.

The detergent may contain 1-65% of a detergent builder or complexing agent such as zeolite, diphosphate, triphosphate, phosphonate, citrate, nitrilotriacetic acid (NTA), ethylene-diami­ne-tetraacetic acid (EDTA), diethylene-triamine-pentaacetic acid (DTMPA), alkyl- or alkenylsuccinic acid, soluble silicates or layered silicates (e.g. SKS-6 from Hoechst). The detergent may also be unbuilt, i.e. essentially free of detergent builder. Compared to a detergent without lipase, the addition of lipase may allow a reduction of the amount of surfactant and/or builder.

The detergent composition may additionally comprise one or more other enzymes, such as amylase, cutinase, protease, cellulase, peroxidase, oxidase, mannanase, pectate lyase, phospholipase.

The enzymes of the detergent composition disclosed herein may be stabilized using conventional stabilizing agents, e.g. a polyol such as propylene glycol or glycerol, a sugar or sugar alcohol, lactic acid, boric acid and borates, derivatives of boric acid and of boronic acid, such as e.g. aromatic esters or substituted boronic acids, in particular (4-formylphenyl)boronic acid. The composition may be formu­lated as described in e.g. WO 92/19709 and WO 92/19708.

The detergent may comprise a lime soap dispersant, e.g. as described in WO 9407985, WO 9504806 or WO 9743379. It may comprise a suitable cationic surfactant such as alkylbenzyldimethylammonium chloride.

The wash temperature may be 10°C, 15 °C, 20 °C, 25°C, 35°C, 40°C, 50 °C or 60 °C.

The pH (measured in aqueous detergent solution at use concentration) may be 6, 7, 8, 9, 10 or 11.

The detergent may be formulated as described in Research Disclosure No. 35944 published on 10 March 1994 b...