Preparation of a Debris-Free Stool Supernatant Suitable for Use with Automated Instruments
Publication Date: 2002-Oct-25
The IP.com Prior Art Database
James A Wolfe Wood: INVENTOR [+3]
A method of producing a debris-free stool supernatant suitable for use with automatic instrumentation is disclosed. A stool sample is briefly centrifuged after mixing with appropriate buffer and/or other diluent. The stool supernatant is then filtered using a serum filter device such as that designed primarily to remove fibrin and particulate matter from serum. The method is applicable to fresh stool samples or to stool samples in various transport media or preservatives.
� � � � � � � � � � � Although enzyme-linked immunoassay (EIA) products applicable to stool specimens are commercially available, stool samples are routinely incompatible for use with automated equipment such as EIA plate or strip washers and EIA plate processors due to occlusion of aspiration probes or tubing with debris common to such specimens.� The problem of occlusion by specimen debris likewise affects other types of automated processes besides EIA.
� � � � � � � � � � � Disclosed below is a method of producing a debris-free stool supernatant suitable for use with instrumentation and considered “automation compatible”.� The method is applicable to fresh untreated stool samples, stool samples in preservative or fixative solutions, or stool samples in other solutions such as microbial transport medium.
1. Prepare the stool sample for testing according to the “Instructions for Use” information provided with the particular diagnostic test kit to be performed.
2. Using standard-sized disposable tubes (10.25 mm, 13 mm, 16 mm, etc.), prepare the desired dilution of a stool sample in the appropriate buffer or solution for the particular test to be performed.
3. Centrifuge the diluted sample at 1500 rpm for 1 – 5 minutes.
4. Select a disposable serum filter device, designed to remove fibrin and particulates from the serum portion of centrifuged blood sampl...