Enzymatic deglycosylation of isoflavones and polydatin by ULTRAFLO TM L and NOVOZYM TM188L into Genistein, Daidzein and Resveratrol
Publication Date: 2002-Nov-26
The IP.com Prior Art Database
Isoflavones are naturally present in soybean and other leguminous plants and reported to have an effect against cancer, as well as other health benefits. The aglycone forms of isoflavones are better absorbed by animals and human beings. ULTRAFLO L and NOVOZYM 188 L, two commercial enzyme preparations from NOVOZYMES A/S, Krogshoejvej 36, DK-2880 Bagsvaerd, Denmark, can deglycosylate isoflavone and resveratrol glucoside into the respective aglycones at a high efficiency under gentle conditions. ULTRAFLO L is a heat-stable multi-active beta-glucanase preparation obtained from a strain of Humicola insolens. The most important activities are beta-glucosidase,cellulase, xylanase, pentosanase and arabanase. NOVOZYM 188 L is obtained by submerged fermentation of a strain of Aspergillus niger and contains cellobiase (beta-1,4-glucosidase) activity. Cellobiase polypeptides described in PCT patent application no. PCT/DK/0200325 filed on May 17, 2002 and claiming priority of DK patent application 2001 00798 of May 18, 2001, are also capable of deglycosylating the compounds described herein. One example of such polypeptide is the cellobiase derived from Aspergillus oryzae strain IFO4177.