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Publication Date: 2003-Jun-30
Document File: 2 page(s) / 38K

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The Prior Art Database

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Novozymes A/S: OWNER

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Use of cutinase for whiteness maintenance

There is a strong need to maintain whiteness of polyester and polyester blend fabric/garment by washing.� � Polyester fabric is hydrophobic and thus it absorbs hydrophobic dirt which makes the� � fabric greyish. Therefore any effective additive to detergents to maintain whiteness will be highly advantageous to position detergent more valuable than others in the market.

We have now found that whiteness maintenance of polyester and polyester blend fabrics after washing is improved by using a detergent containing cutinase.

The cutinase may be acting on amorphous part of polyester fiber and thus it prevents deposition of dirt or removes deposited dirt.

The cutinase may be a fungal cutinase, e.g. the cutinases of Fusarium solani pisi (S. Longhi et al., Journal of Molecular Biology, 268 (4), 779-799 (1997)) or Humicola insolens (US 5,827,719). Alternatively, the cutinase may be a bacterial cutinase, e.g. from Pseudomonas such as P. mendocina (US 5,389,536) or P. putida (WO 88/09367). Further, the cutinase may be a variant, e.g. as described in WO 0034450, WO 0192502, WO 9414963 or WO 9414964.

The detergent may further comprise other enzymes and/or detergent components such as surfactants, anti-redeposition agents, optical brighteners, bleaching agents, builders, fabric coating agents etc.

Experimental data

The effect of a cutinase was demonstrated in the following experiments. White polyester textiles was washed with cutinase and Japanese commercial detergent under Japanese standard conditions (25°C, 3 dH, 10 min, 100 rpm by Terg-o-Tometer) several times with and without particle soils (carbon black and ferric oxide).

The results are shown as reflectance (Z value) after 7 wash cycles with soil:


reflectance (Z value)

Standard deviation

Polyester A






Enzyme treated



Polyester B






Enzyme treated



It was found that enzyme treated polyester textile had higher reflectance than that of control experiment.

Detergent Composition

The cutinase is typically incorporated in the detergent composition at a level from 0.00001% to 2% of enzyme protein by weight of the composition, particularly at a level from 0.0001% to 1% of enzyme protein by weight of the composition, more pa...