Browse Prior Art Database

USE OF CLOUD POINT EXTRACTION

IP.com Disclosure Number: IPCOM000021761D
Publication Date: 2004-Feb-06

Publishing Venue

The IP.com Prior Art Database

Abstract

A method of extracting a polypeptide of interest from a fermentation broth comprising: i) adjusting the pH close to pI of the polypeptide of interest; ii) adding a non-ionic surfactant with a hydrophile-lipophile balance (HLB) of 12 or lower; iii) cooling the mixture for solubilization and incubating at above cloud point for extraction; iv) phase separating at below cloud point to obtain liquid-liquid-solid fractions; and v) recovering the surfactant-rich top phase containing the polypeptide of interest.