Color Coding Cluster Sizes From Electron Microscope Images
Original Publication Date: 1984-Sep-01
Included in the Prior Art Database: 2005-Feb-05
Techniques are available for assigning particles of a given size from electron microscope images, a specific image density, and hence color. However, the number of objects analyzed is usually very limited (20 or less). Large numbers of particles can be color coded in descending order of size or some other particle feature (such as maximum cord length, x or y, axis projection, equivalent spherical diameter, etc.) if a cluster analysis is performed on a digitized image. In this technique, the electron microscope image is first digitized, after which a cluster analysis is performed on the digitized image. The particles are then sorted by size or particle feature, in a descending order via a computer program. An algorithm for grey levels, represented by the following expression 254 Sin(N-1)r/2 +1 is then applied.