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Transgenic lead production and early evaluation Disclosure Number: IPCOM000242554D
Publication Date: 2015-Jul-24
Document File: 2 page(s) / 18K

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Pioneer Hi-Bred International, Inc.: AUTHOR

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Transgenic lead production and early evaluation

Faster and more efficient event production is a benefit for commercial seed product development.  Timing and advancement of lead events is often limited by many factors, including but not limited to time in tissue culture, rate of plant development, and general mechanical process limitations.  Nevertheless, there are many tools available to researchers to accelerate the timelines.

For example, a process for faster development can be envisioned as follows. 

Constructs are created by vector construction teams to fill event production pipelines (1).  Prior to entering the transformation pipeline all constructs pass a vector quality control step.  Transformation groups produce different amounts of raw events per construct depending on trait gene targets (2).  T0 plants are delivered to a plant husbandry group that uses various controlled environment tools to maintain good plant health of the delivered plantlets (3).  Once in controlled environments, the T0 plants get evaluated for molecular quality such as qPCR.  This data is used to cull plants that do not meet specified molecular criteria.  The quality-enriched T0 plants are then punched for southern by sequencing (SBS) or other testing (4).  With each new assay the T0 population is further enriched for molecular quality. 

The resulting enriched population of T0s can be used for multiple purposes to drive a faster event production pipeline.  Their pollen can be used in a multitude of different pollination schemes (5). 

·         SELF’ed T0 plants will make 1:2:1 T1 seed for further testing

·         T0 pollen may be carried out to any number of genetic targets making 1:1 T1 seed for further testing

·         Pollen...