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Publication Date: 2016-Jun-03

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The Prior Art Database

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Novozymes A/S: OWNER


The present invention concerns an assay and a method for testing the presence of a fluorophore on a first and/or a second surface, wherein several different compositions are prepared with fluorophores having different absorbance and emission wavelengths.

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     The present invention concerns an assay and a method for testing the presence of a fluorophore on a first and/or a second surface. The invention further concerns an item with a surface comprising the composition and a composition comprising a fluorophore. The invention is based on the disclosure in

IPCOM000242729D, which is hereby incorporated by reference.

Description of the Related Art

     A fluorophore absorbs light energy of a specific wavelength and re-emits light at a longer wavelength. The wavelengths of the absorbed light, energy transfer efficiency, and time before emission depend on both

the fluorophore structure and its chemical environment, as the molecule in its excited state interacts with surrounding molecules. Wavelengths of maximum absorption (≈ excitation) and maximum emission (for example, Absorption/Emission = 485 nm/517 nm) are the typical terms used to refer to a given fluorophore, but the whole spectrum may be important to consider.

Excitation energies range from ultraviolet through the visible spectrum, and emission energies may

continue from visible light into the near infrared region.

     Wash performance has traditionally been addressed by use of remission values where an item after wash is submitted to light at 460 nm and the remission of light is measured and can be compared to the remission before wash as described in WO 2002/099091. However, for some stains it is not possible to see the stain by human eye after wash. The item may appear clean after wash, even though the stain or residuals

of the stain may still be present on the item. It is therefore very difficult to detect if an item is completely clean or if a part of the stain is still present after wash.


Absorbance wavelength: By the term "absorbance wavelength" is understood the wavelength, where

the fluorophore has its highest absorbtion (corresponding to the peak in the absorption spectrum of the fluorophore).

     Composition for cleaning hard surfaces: The term "composition for cleaning hard surfaces" refers to compositions intended for cleaning hard surfaces such as floors, tables, walls, roofs etc. as well as surfaces of hard objects such as cars (car wash) and dishes (dishware).

     Dishware: The term "dishware" is intended to mean any form of kitchen utensil used in domestic or industrial kitchen or food industry such as dinner set or tableware such as but not limited to pans, plates, cops, knives, forks, spoons, porcelain etc. The dishware can be made of any suitable material such as metal, glass, rubber, plastic, PVC, acrylics, ceramics, china or porcelain.

Dish washing composition: The term "dish washing composition" refers to compositions comprising

detergent components, which composition is intended for cleaning dishes, table ware, glass ware, cutting boards, pots, pans, cutlery and all forms of compositions for cleaning hard surfaces areas in kitchens. The present invention is not restricted to any part...