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Publication Date: 2017-Apr-12

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The Prior Art Database

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Enzymes have been used in detergents for decades. Usually a cocktail of various enzymes is

added to detergent compositions. The enzyme cocktail often comprises various enzymes, 5

wherein each enzyme targets it specific substrate e.g. amylases are active towards starch stains,

proteases on protein stains and so forth. Textiles surface and hard surfaces, such as dishes or

the inner space of a laundry machine enduring several wash cycles, become soiled with many

different types of soiling which may compose of proteins, grease, starch etc. One type of soiling

may be organic matter, such as biofilm, extracellular polymeric substance (EPS), etc. Organic 10

matter composes different molecules such as polysaccharides, extracellular DNA (eDNA), and

proteins. Some organic matter composes an extracellular polymeric matrix, which may be sticky

or glueing, which when present on textile, attracts soils and may course redeposition or

backstaining of soil resulting in a greying of the textile. Additionally, organic matters such as

biofilms often cause malodor issue as various malodor molecules can be adhered by the 15

polysaccharides, extracellular DNA (eDNA), and proteins in the complex extracellular matrix and

be slowly released out to cause consumer noticeable malodor issue. There is a need for cleaning

compositions, which effectively prevent, reduce or remove components of organic soiling.

Detailed Description20

Various enzymes are applied in cleaning processes each targeting specific types of soiling such

as protein, starch and grease soiling. Enzymes are now standard ingredients in detergents for

laundry and dish wash. The effectiveness of these commercial enzymes provides detergents

which removes much of the soiling. However, organic matters such as EPS (extracellular

polymeric substance) comprised in much biofilm constitute a challenging type of soiling due to 25

the complex nature of such organic matters. None of the commercially available cleaning

compositions effectively remove or reduce EPS and/or biofilm related soiling. Biofilm may be

produced when a group of microorganisms’ cells stick to each other or stick to a surface, such as

a textile, dishware or hard surface or another kind of surface. These adherent cells are frequently

embedded within a self-produced matrix of extracellular polymeric substance (EPS), which30

constitute 50% to 90% of the biofilm's total organic matter. EPS is mostly composed of

polysaccharides (exopolysaccharides) and proteins, but include other macro-molecules such as

eDNA, lipids and other organic substances. Organic matter like biofilm may be sticky or glueing,

which when present on textile, may give rise to redeposition or backstaining of soil resulting in a

greying of the textile. Another drawback of organic matter e.g. biofilm is the malodor as various 35

malodor related molecules are often associated with organic matter e.g. biofilm. Further, when

dirty laundry...