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Enzymes in CIP Disclosure Number: IPCOM000249971D
Publication Date: 2017-May-11
Document File: 6 page(s) / 1M

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The Prior Art Database

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Use of protease for cleaning-in-place, cleaning-out-of-place and cleaning of open places.

Proteases (PRO01-PRO09) are suitable for cleaning of all places which have proteinaceous soils. Since such soils

are rarely consisting of only proteins the proteases may be combined with other enzymes, such as amylases

and/or lipases when cleaning such places. Places with proteinaceous soils to be cleaned include but are not

limited to closed systems, open places and places and equipment which can be disassembled and cleaned out-

of-place. Closed systems include, but are not limited to pipes, tanks, heat exchangers and membranes in

industries which are processing liquids including milk and dairy products, and juices. Also closed equipment in

fermentation based industries like brewing, sauces (soy sauce etc.) biotech, cultures and pharma are included.

Open places with proteinaceous soilings are found in industries including, but are not limited to the meat,

poultry and fish processing industry, the fruit and vegetable processing plants, bakeries, and cake and

confectionary manufacturing. Relevant open places include, but are not limited to floors, walls and drains,

conveyor belts, cutting boards, trays and knives. Examples of equipment which can be disassembled for

cleaning include cutting equipment in the meat processing industry, and injector needle systems for curing and

marinating all types of meat, poultry and fish products.

Assay Description – Cleaning of ultrafiltration membranes fouled with whey protein

In order to assess the cleaning performance of PR003 on ultrafiltration membranes fouled with whey protein, a

cleaning performance test is performed using the Labstak® M10 ultrafiltration membrane system from Alfa

Laval. The Labstak® M10 system is equipped with two GR80PP polyethersulfone membranes of 10,000 Dalton

MWCO, and is a table-top model of a crossflow filtration unit. Prior to cleaning, the membranes are fouled with

whey protein by recirculating a whey protein solution over the membranes for 45 minutes. Before, during and

after cleaning, the membrane flux rate in liter/hour/m2 is calculated by continuously gravimetrically measuring

the permeate flux stream. Furthermore, samples are collected from retentate and permeate solutions

throughout the experiment in order to follow possible protein build up in the system as well as possible protein

degradation. The protein content as well as the protein degradation profile is analyzed using Sodium Dodecyl

Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE). The cleaning performance is evaluated by comparison

of the flux rate of the final water rinse after cleaning and comparison of the protein detected in the samples

collected during and after cleaning from the retentate and permeate solutions.

Example - Cleaning of ultrafiltration membranes fouled with whey protein:

Using the membrane system Labstak® M10 from Alfa Laval, equipped with two GR80PP polyethersulfone

membranes of 10,000 Dalton MWCO,...